|Application ||WB, E|
|Other Accession||Q8BGE6, Q6DG88, Q6PZ02, Q6PZ03|
|Predicted||Bovine, Chicken, Zebrafish, Mouse|
|Calculated MW||44294 Da|
|Antigen Region||250-290 aa|
|Other Names||Cysteine protease ATG4B, 3422-, AUT-like 1 cysteine endopeptidase, Autophagin-1, Autophagy-related cysteine endopeptidase 1, Autophagy-related protein 4 homolog B, hAPG4B, ATG4B, APG4B, AUTL1, KIAA0943|
|Target/Specificity||This ATG4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-290 amino acids from the Central region of human ATG4B.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ATG4B Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||APG4B, AUTL1, KIAA0943|
|Function||Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C- terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.|
|Tissue Location||Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas|
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Provided below are standard protocols that you may find useful for product applications.
Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
Marino G., et al. J. Biol. Chem. 278:3671-3678(2003).
Kabeya Y., et al. J. Cell Sci. 117:2805-2812(2004).
Nagase T., et al. DNA Res. 6:63-70(1999).
Ohara O., et al. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
Ota T., et al. Nat. Genet. 36:40-45(2004).
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