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POLH Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - POLH Antibody (N-term) AP20546a
    POLH Antibody (N-term) (Cat.# AP20546a) western blot analysis in CEM cell lysate (35ug/lane). This demonstrates that the POLH antibody detected the POLH protein (arrow).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9Y253
Other Accession Q9JJN0
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 78413 Da
Additional Information
Gene ID 5429
Other Names DNA polymerase eta, RAD30 homolog A, Xeroderma pigmentosum variant type protein, POLH, RAD30, RAD30A, XPV
Target/Specificity This POLH antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 68-92 amino acids of human POLH.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPOLH Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name POLH
Synonyms RAD30, RAD30A, XPV
Function DNA polymerase specifically involved in the DNA repair by translesion synthesis (TLS) (PubMed:10385124, PubMed:11743006, PubMed:24449906). Due to low processivity on both damaged and normal DNA, cooperates with the heterotetrameric (REV3L, REV7, POLD2 and POLD3) POLZ complex for complete bypass of DNA lesions. Inserts one or 2 nucleotide(s) opposite the lesion, the primer is further extended by the tetrameric POLZ complex. In the case of 1,2-intrastrand d(GpG)-cisplatin cross-link, inserts dCTP opposite the 3' guanine (PubMed:24449906). Particularly important for the repair of UV-induced pyrimidine dimers (PubMed:10385124, PubMed:11743006). Although inserts the correct base, may cause base transitions and transversions depending upon the context. May play a role in hypermutation at immunoglobulin genes (PubMed:11376341, PubMed:14734526). Forms a Schiff base with 5'- deoxyribose phosphate at abasic sites, but does not have any lyase activity, preventing the release of the 5'-deoxyribose phosphate (5'-dRP) residue. This covalent trapping of the enzyme by the 5'- dRP residue inhibits its DNA synthetic activity during base excision repair, thereby avoiding high incidence of mutagenesis (PubMed:14630940). Targets POLI to replication foci (PubMed:12606586).
Cellular Location Nucleus. Note=Accumulates at replication forks after DNA damage (PubMed:12606586). After UV irradiation, recruited to DNA damage sites within 1 hour, to a maximum of about 80%; this recruitment may not be not restricted to cells active in DNA replication (PubMed:22801543).
Citations (0)

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Background

DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Plays an important role in the repair of UV-induced pyrimidine dimers. Depending on the context, it inserts the correct base, but causes frequent base transitions and transversions. May play a role in hypermutation at immunoglobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity. Targets POLI to replication foci.

References

Masutani C.,et al.Nature 399:700-704(1999). Johnson R.E.,et al.Science 285:263-265(1999). Yuasa M.,et al.Oncogene 19:4721-4728(2000). Mungall A.J.,et al.Nature 425:805-811(2003). Glick E.,et al.EMBO J. 20:7303-7312(2001).

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Discontinued
Cat# AP20546a
Size:
(40 western blots)

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