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POLH Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - POLH Antibody (N-term) AP20546a
    POLH Antibody (N-term) (Cat.# AP20546a) western blot analysis in CEM cell lysate (35ug/lane). This demonstrates that the POLH antibody detected the POLH protein (arrow).
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9Y253
Other Accession Q9JJN0
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 78413 Da
Additional Information
Gene ID 5429
Other Names DNA polymerase eta, RAD30 homolog A, Xeroderma pigmentosum variant type protein, POLH, RAD30, RAD30A, XPV
Target/Specificity This POLH antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 68-92 amino acids from the N-terminal region of human POLH.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPOLH Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name POLH
Synonyms RAD30, RAD30A, XPV
Function DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Plays an important role in the repair of UV-induced pyrimidine dimers. Depending on the context, it inserts the correct base, but causes frequent base transitions and transversions. May play a role in hypermutation at immunoglobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity. Targets POLI to replication foci.
Cellular Location Nucleus. Note=Accumulates at replication forks after DNA damage
Research Areas
Citations (0)

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Background

DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Plays an important role in the repair of UV-induced pyrimidine dimers. Depending on the context, it inserts the correct base, but causes frequent base transitions and transversions. May play a role in hypermutation at immunoglobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity. Targets POLI to replication foci.

References

Masutani C.,et al.Nature 399:700-704(1999). Johnson R.E.,et al.Science 285:263-265(1999). Yuasa M.,et al.Oncogene 19:4721-4728(2000). Mungall A.J.,et al.Nature 425:805-811(2003). Glick E.,et al.EMBO J. 20:7303-7312(2001).

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$ 295.00
$ 99.00
Cat# AP20546a
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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