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POLR2J3 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - POLR2J3 Antibody (N-term) AP20548a
    Western blot analysis of lysate from MCF-7 cell line,using POLR2J3 Antibody (N-term)(Cat. #AP20548a).AP20548a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane.
  • SPECIFICATION
  • CITATIONS
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  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9H1A7
Other Accession Q9GZM3
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 13092 Da
Additional Information
Other Names DNA-directed RNA polymerase II subunit RPB11-b2, RNA polymerase II subunit B11-b2, RPB11b2, DNA-directed RNA polymerase II subunit J3, POLR2J3
Target/Specificity This POLR2J3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1-25 amino acids from the N-terminal region of human POLR2J3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPOLR2J3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name POLR2J3
Function DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB11 is part of the core element with the central large cleft (By similarity).
Cellular Location Nucleus.
Research Areas
Citations (0)

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Background

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB11 is part of the core element with the central large cleft (By similarity).

References

Grandemange S.,et al.BMC Mol. Biol. 2:14-14(2001).
Hillier L.W.,et al.Nature 424:157-164(2003).

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$ 295.00
$ 99.00
Cat# AP20548a
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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