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MYH14 Antibody (Center)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | Q7Z406 |
| Other Accession | Q6URW6 |
| Reactivity | Human |
| Predicted | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 227871 Da |
| Antigen Region | 654-668 aa |
| Gene ID | 79784 |
|---|---|
| Other Names | Myosin-14, Myosin heavy chain 14, Myosin heavy chain, non-muscle IIc, Non-muscle myosin heavy chain IIc, NMHC II-C, MYH14, KIAA2034 |
| Target/Specificity | This MYH14 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 654-668 amino acids from the Central region of human MYH14. |
| Dilution | WB~~1:1000 IHC-P~~1:25 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | MYH14 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | MYH14 |
|---|---|
| Synonyms | KIAA2034 |
| Function | Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. |
| Tissue Location | High levels of expression are found in brain (highest in corpus callosum), heart, kidney, liver, lung, small intestine, colon and skeletal muscle. Expression is low in organs composed mainly of smooth muscle, such as aorta, uterus and urinary bladder. No detectable expression is found in thymus, spleen, placenta and lymphocytes. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping (By similarity).
References
Leal A.,et al.Gene 312:165-171(2003).
Nagase T.,et al.Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases.
Grimwood J.,et al.Nature 428:529-535(2004).
Bienvenut W.V.,et al.Submitted (NOV-2006) to UniProtKB.
Jana S.S.,et al.J. Biol. Chem. 284:11563-11571(2009).
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If you have any additional inquiries please email technical services at tech@abcepta.com.
Anonymous
2016-08-23 21:15:32
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Species tested
Human,Mouse,Rat
Application tested
WB
Brief protocol
1. Block with 3% skim milk for 1 hour at room temperature.
2. Incubate overnight with Abgent primary antibody 1:1000 in 3% skim milk at 4℃
3. Wash 5*5 min with TBST.
4. Incubate with HRP-conjugated secondary antibody 1:5000 in 3% skim milk for 1 hour at room temperature.
5. Wash 5*5 min with TBST.
6. Incubate with ECL substrates and expose |
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