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ALDH1A1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IF - ALDH1A1 Antibody (Center) AP20580c
    Fluorescent image of A549 cells stained with ALDH1A1 Antibody (Center)(Cat#AP20580c). AP20580c was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
  • WB - ALDH1A1 Antibody (Center) AP20580c
    Western blot analysis of lysates from A549 cell line and rat liver tissue lysate (from left to right), using ALDH1A1 Antibody (Center) (Cat. #AP20580c). AP20580c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession P00352
Other Accession Q8HYE4
Reactivity Human, Rat
Predicted Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 54862 Da
Additional Information
Gene ID 216
Other Names Retinal dehydrogenase 1, RALDH 1, RalDH1, ALDH-E1, ALHDII, Aldehyde dehydrogenase family 1 member A1, Aldehyde dehydrogenase, cytosolic, ALDH1A1, ALDC, ALDH1, PUMB1
Target/Specificity This ALDH1A1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 306-339 amino acids from the Central region of human ALDH1A1.
Dilution IF~~1:25
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsALDH1A1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ALDH1A1 (HGNC:402)
Function Can convert/oxidize retinaldehyde to retinoic acid. Binds free retinal and cellular retinol-binding protein-bound retinal (By similarity). May have a broader specificity and oxidize other aldehydes in vivo (PubMed:19296407, PubMed:26373694, PubMed:25450233).
Cellular Location Cytoplasm, cytosol {ECO:0000250|UniProtKB:P48644}
Research Areas
Citations (0)

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Background

Binds free retinal and cellular retinol-binding protein- bound retinal. Can convert/oxidize retinaldehyde to retinoic acid (By similarity).

References

Hsu L.C.,et al.Genomics 5:857-865(1989).
Zheng C.F.,et al.Alcohol. Clin. Exp. Res. 17:828-831(1993).
Ramana K.V.,et al.Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases.
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Humphray S.J.,et al.Nature 429:369-374(2004).

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$ 295.00
$ 99.00
Cat# AP20580c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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