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>   home   >   Products   >   Primary Antibodies   >   Metabolism   >   IL18 Antibody (C-term)   

IL18 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IHC-P - IL18 Antibody (C-term) AP20583c
    Immunohistochemical analysis of paraffin-embedded H. small intestine section using IL18 Antibody (C-term)(Cat#AP20583c). AP20583c was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • IF - IL18 Antibody (C-term) AP20583c
    Fluorescent image of U251 cells stained with IL18 Antibody (C-term) (Cat#AP20583c). AP20583c was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
  • FC - IL18 Antibody (C-term) AP20583c
    Flow cytometric analysis of Hela cells using IL18 Antibody (C-term)(green, Cat#AP20583c) compared to an isotype control of rabbit IgG(blue). AP20583c was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
  • IHC-P - IL18 Antibody (C-term) AP20583c
    Immunohistochemical analysis of paraffin-embedded R. small intestine section using IL18 Antibody (C-term)(Cat#AP20583c). AP20583c was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • IHC-P - IL18 Antibody (C-term) AP20583c
    Immunohistochemical analysis of paraffin-embedded M. small intestine section using IL18 Antibody (C-term)(Cat#AP20583c). AP20583c was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • WB - IL18 Antibody (C-term) AP20583c
    Western blot analysis of lysate from Hela cell line, using IL18 Antibody (C-term) (Cat. #AP20583c). AP20583c was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, FC, E
Primary Accession Q14116
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 22326 Da
Additional Information
Gene ID 3606
Other Names Interleukin-18, IL-18, Iboctadekin, Interferon gamma-inducing factor, IFN-gamma-inducing factor, Interleukin-1 gamma, IL-1 gamma, IL18, IGIF, IL1F4
Target/Specificity This IL18 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 155-188 amino acids from the C-terminal region of human IL18.
Dilution IHC-P~~1:25
IF~~1:25
FC~~1:25
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIL18 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IL18
Synonyms IGIF, IL1F4
Function Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.
Cellular Location Secreted.
Research Areas
Citations (0)

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Background

Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.

References

Ushio S.,et al.J. Immunol. 156:4274-4279(1996).
Gaggero A.,et al.Oncogene 23:7552-7560(2004).
Yong D.,et al.Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases.
Liu J.,et al.Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.

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Discontinued
Cat# AP20583c
Size:
(40 western blots)
Alternative Products:

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