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HIST1H4A Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - HIST1H4A Antibody (C-term) AP20585c
    All lanes : Anti-HIST1H4A Antibody (C-term) at 1:2000 dilution Lane 1: HL-60 whole cell lysates Lane 2: NIH/3T3 whole cell lysates Lane 3: Hela whole cell lysates Lane 4: A431 whole cell lysates Lane 5: H-4-Ⅱ-E whole cell lysates Lane 6: C6 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 11 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - HIST1H4A Antibody (C-term) AP20585c
    Immunohistochemical analysis of paraffin-embedded H. skin section using HIST1H4A Antibody (C-term)(Cat#AP20585c). AP20585c was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • FC - HIST1H4A Antibody (C-term) AP20585c
    Flow cytometric analysis of MCF-7 cells using HIST1H4A Antibody (C-term)(green, Cat#AP20585c) compared to an isotype control of rabbit IgG(blue). AP20585c was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
  • IHC-P - HIST1H4A Antibody (C-term) AP20585c
    Immunohistochemical analysis of paraffin-embedded R. skin section using HIST1H4A Antibody (C-term)(Cat#AP20585c). AP20585c was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • IHC-P - HIST1H4A Antibody (C-term) AP20585c
    Immunohistochemical analysis of paraffin-embedded M. skin section using HIST1H4A Antibody (C-term)(Cat#AP20585c). AP20585c was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • IHC-P - HIST1H4A Antibody (C-term) AP20585c
    Immunohistochemical analysis of paraffin-embedded H. esophagus section using HIST1H4A Antibody (C-term)(Cat#AP20585c). AP20585c was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • IHC-P - HIST1H4A Antibody (C-term) AP20585c
    Immunohistochemical analysis of paraffin-embedded M. esophagus section using HIST1H4A Antibody (C-term)(Cat#AP20585c). AP20585c was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • WB - HIST1H4A Antibody (C-term) AP20585c
    Western blot analysis of lysates from A431, Hela, H-4-II-E, L929 cell lines (from left to right), using HIST1H4A Antibody (C-term) (Cat. #AP20585c). AP20585c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession P62805
Other Accession P02309, P62799, P62804, P62802, P62806, Q4R362, P84040, P62801, P62784, P62803
Reactivity Human, Mouse, Rat
Predicted Rat, Monkey, Pig, Chicken, Yeast, Xenopus, Bovine, C.Elegans, Drosophila
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 11367 Da
Additional Information
Gene ID 121504;554313;8294;8359;8360;8361;8362;8363;8364;8365;8366;8367;8368;8370
Other Names Histone H4, HIST1H4A, H4/A, H4FA
Target/Specificity This HIST1H4A antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 71-103 amino acids from the C-terminal region of human HIST1H4A.
Dilution WB~~1:1000
IHC-P~~1:25
FC~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHIST1H4A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HIST1H4A
Synonyms H4/A, H4FA
Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Cellular Location Nucleus. Chromosome.
Research Areas
Citations (0)

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Background

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

References

Sierra F.,et al.Nucleic Acids Res. 11:7069-7086(1983).
Pauli U.,et al.Science 236:1308-1311(1987).
Albig W.,et al.Genomics 10:940-948(1991).
Drabent B.,et al.DNA Cell Biol. 14:591-597(1995).
Albig W.,et al.Gene 184:141-148(1997).

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Discontinued
Cat# AP20585c
Size:
(40 western blots)
Alternative Products:

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