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AGAP4 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IF - AGAP4 Antibody (C-term) AP20603c
    Fluorescent image of HeLa cells stained with AGAP4 Antibody (C-term)(Cat#AP20603c). AP20603c was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
    detail
  • WB - AGAP4 Antibody (C-term) AP20603c
    Western blot analysis of lysate from HT-1080 cell line, using AGAP4 Antibody (C-term)(Cat. # AP20603c). AP20603c was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
    detail
  • IHC-P - AGAP4 Antibody (C-term) AP20603c
    Immunohistochemical analysis of paraffin-embedded H. liver section using AGAP4 Antibody (C-term)(Cat#AP20603c). AP20603c was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
    detail
  • IHC-P - AGAP4 Antibody (C-term) AP20603c
    Immunohistochemical analysis of paraffin-embedded M. liver section using AGAP4 Antibody (C-term)(Cat#AP20603c). AP20603c was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
    detail
  • FC - AGAP4 Antibody (C-term) AP20603c
    Flow cytometric analysis of MCF-7 cells using AGAP4 Antibody (C-term)(green, Cat#AP20603c) compared to an isotype control of rabbit IgG(blue). AP20603c was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, FC, E
Primary Accession Q96P64
Other Accession Q8TF27, A6NIR3, Q5VW22, Q5VTM2
Reactivity Human
Predicted Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 73070 Da
Additional Information
Gene ID 119016
Other Names Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 4, Centaurin-gamma-like family member 1, Centaurin-gamma-like family member 5, AGAP8, CTGLF1, CTGLF5, MRIP2
Target/Specificity This AGAP4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 498-531 amino acids from the C-terminal region of human AGAP4
Dilution IF~~1:25
WB~~1:1000
IHC-P~~1:25
FC~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAGAP4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AGAP4 (HGNC:23459)
Function Putative GTPase-activating protein.
Research Areas
Citations (0)
citation

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Background

Putative GTPase-activating protein (Potential).

References

Deloukas P.,et al.Nature 429:375-381(2004).
Varela I.,et al.Nature 469:539-542(2011).

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$ 365.00
$ 140.00
Cat# AP20603c
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