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DHFR Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IHC-P - DHFR Antibody (N-term) AP20626a
    Immunohistochemical analysis of paraffin-embedded H. liver section using DHFR Antibody (N-term)(Cat#AP20626a). AP20626a was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
  • IHC-P - DHFR Antibody (N-term) AP20626a
    Immunohistochemical analysis of paraffin-embedded H. brain section using DHFR Antibody (N-term)(Cat#AP20626a). AP20626a was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
  • WB - DHFR Antibody (N-term) AP20626a
    Western blot analysis of lysates from 293T, Hela, Jurkat, Y79 cell line , mouse spleen and rat kidney tissue lysate(from left to right), using DHFR Antibody (N-term)(Cat. #AP20626a). AP20626a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P00374
Other Accession P00377, P00375, P00376
Reactivity Human, Mouse, Rat
Predicted Bovine, Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 21453 Da
Additional Information
Gene ID 1719
Other Names Dihydrofolate reductase, DHFR
Target/Specificity This DHFR antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 16-30 amino acids from the N-terminal region of human DHFR.
Dilution IHC-P~~1:25
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDHFR Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DHFR
Function Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1.
Tissue Location Widely expressed in fetal and adult tissues, including throughout the fetal and adult brains and whole blood Expression is higher in the adult brain than in the fetal brain
Research Areas
Citations (0)

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Background

Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1.

References

Chen M.-J.,et al.J. Biol. Chem. 259:3933-3943(1984).
Masters J.N.,et al.Gene 21:59-63(1983).
Yang J.K.,et al.J. Mol. Biol. 176:169-187(1984).
Schmutz J.,et al.Nature 431:268-274(2004).
Banka S.,et al.Am. J. Hum. Genet. 88:216-225(2011).

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$ 295.00
$ 99.00
Cat# AP20626a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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