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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ETFA Antibody (Center)   

ETFA Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - ETFA Antibody (Center) AP20630c
    Fluorescent image of Hela cells stained with ETFA Antibody (Center)(Cat#AP20630c). AP20630c was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
  • WB - ETFA Antibody (Center) AP20630c
    Western blot analysis of lysates from A431, Hela, HepG2 cell line , human liver and mouse liver tissue lysate(from left to right), using ETFA Antibody (Center)(Cat. #AP20630c). AP20630c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession P13804
Other Accession Q8HXY0
Reactivity Human, Mouse
Predicted Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 35080 Da
Additional Information
Gene ID 2108
Other Names Electron transfer flavoprotein subunit alpha, mitochondrial, Alpha-ETF, ETFA
Target/Specificity This ETFA antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 192-226 amino acids from the Central region of human ETFA.
Dilution IF~~1:25
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsETFA Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ETFA
Function The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl- CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).
Cellular Location Mitochondrion matrix.
Research Areas
Citations (0)

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Background

The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl- CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).

References

Finocchiaro G.,et al.J. Biol. Chem. 263:15773-15780(1988).
Olsen R.K.J.,et al.Hum. Mutat. 22:12-23(2003).
Kalnine N.,et al.Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Zody M.C.,et al.Nature 440:671-675(2006).

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$ 295.00
$ 99.00
Cat# AP20630c
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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