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TUFM Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

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  • IF - TUFM Antibody (N-term) AP20640a
    Fluorescent image of Hela cells stained with TUFM Antibody (N-term)(Cat#AP20640a). AP20640a was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
  • WB - TUFM Antibody (N-term) AP20640a
    Western blot analysis of lysate from A431 cell line, using TUFM Antibody (N-term)(Cat. #AP20640a). AP20640a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
  • WB - TUFM Antibody (N-term) AP20640a
    Western blot analysis of lysates from HepG2, Hela, mouse NIH/3T3 cell line and rat brain tissue lysate(from left to right), using TUFM Antibody (N-term)(Cat. #AP20640a). AP20640a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P49411
Other Accession P85834, Q8BFR5, P49410
Reactivity Human, Mouse, Rat
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 49542 Da
Additional Information
Gene ID 7284
Other Names Elongation factor Tu, mitochondrial, EF-Tu, P43, TUFM
Target/Specificity This TUFM antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 112-147 amino acids from the N-terminal region of human TUFM.
Dilution IF~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTUFM Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
Cellular Location Mitochondrion.
Research Areas
Citations (0)

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This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.


Woriax V.L.,et al.Biochim. Biophys. Acta 1264:347-356(1995).
Wells J.,et al.FEBS Lett. 358:119-125(1995).
Ling M.,et al.Gene 197:325-336(1997).
Martin J.,et al.Nature 432:988-994(2004).
Dunn M.J.,et al.Submitted (MAR-1996) to UniProtKB.

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$ 295.00
$ 99.00
Cat# AP20640a
(40 western blots)
Availability: In Stock
Bulk Size
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