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Smad1/5 (Ser463/465) Antibody

Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - Smad1/5 (Ser463/465) Antibody AP20641b
    Western blot analysis of lysates from HepG2, HT-1080 cell line (from left to right), using Smad1/5 Antibody (Ser463/465). ctrl3(Cat. #AP20641b). AP20641b was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q15797
Other Accession O54835, Q9JIW5, O15198, P97588, P70340, Q9I8V2, Q1JQA2
Reactivity Human
Predicted Bovine, Zebrafish, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 52260 Da
Additional Information
Gene ID 4086
Other Names Mothers against decapentaplegic homolog 1, MAD homolog 1, Mothers against DPP homolog 1, JV4-1, Mad-related protein 1, SMAD family member 1, SMAD 1, Smad1, hSMAD1, Transforming growth factor-beta-signaling protein 1, BSP-1, SMAD1, BSP1, MADH1, MADR1
Target/Specificity This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 455-485 amino acids from human.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSmad1/5 (Ser463/465) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SMAD1
Synonyms BSP1, MADH1, MADR1
Function Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression.
Cellular Location Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane
Tissue Location Ubiquitous. Highest expression seen in the heart and skeletal muscle
Research Areas
Citations (0)

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Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.


Riggins G.J.,et al.Nat. Genet. 13:347-349(1996).
Liu F.,et al.Nature 381:620-623(1996).
Hoodless P.A.,et al.Cell 85:489-500(1996).
Lechleider R.J.,et al.J. Biol. Chem. 271:17617-17620(1996).
Zhang Y.,et al.Nature 383:168-172(1996).

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$ 295.00
$ 99.00
Cat# AP20641b
(40 western blots)
Availability: In Stock
Bulk Size
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