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>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   CERKL Antibody (N-term)   

CERKL Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CERKL Antibody (N-term) AP20663a
    Western blot analysis of lysate from human normal uterus tissue lysate, using CERKL Antibody (N-term)(Cat. #AP20663a). AP20663a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
  • WB - CERKL Antibody (N-term) AP20663a
    Western blot analysis of lysate from 293 cell line, using CERKL Antibody (N-term)(Cat. #AP20663a). AP20663a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q49MI3
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 62622 Da
Additional Information
Gene ID 375298
Other Names Ceramide kinase-like protein, CERKL
Target/Specificity This CERKL antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 94-127 amino acids from the N-terminal region of human CERKL.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCERKL Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CERKL
Function Has no detectable ceramide-kinase activity. Overexpression of CERKL protects cells from apoptosis in oxidative stress conditions.
Cellular Location Cytoplasm. Nucleus, nucleolus. Note=Enriched in nucleoli. May shuttle between nucleus and cytoplasm. Isoform 5 is not enriched in the nucleoli
Tissue Location Isoform 1 and isoform 2 are expressed in adult retina, liver and pancreas as well as in fetal brain, lung and kidney. Isoform 3 is expressed in adult retina as well as in fetal lung and liver. Isoform 4 is expressed in adult retina, lung and kidney as well as in fetal lung and liver. Moderately expressed in retina, kidney, lung, testis, trachea, and pancreas. Weakly expressed in brain, placenta and liver
Research Areas
Citations (0)

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Background

Has no detectable ceramide-kinase activity. Overexpression of CERKL protects cells from apoptosis in oxidative stress conditions.

References

Tuson M.,et al.Am. J. Hum. Genet. 74:128-138(2004).
Bornancin F.,et al.Biochim. Biophys. Acta 1687:31-43(2005).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Hillier L.W.,et al.Nature 434:724-731(2005).
Rovina P.,et al.Biochim. Biophys. Acta 1791:1023-1030(2009).

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$ 295.00
$ 99.00
Cat# AP20663a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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