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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Mouse Hck Antibody (Center)   

Mouse Hck Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - Mouse Hck Antibody (Center) AP20682c
    Western blot analysis of lysate from mouse thymus and rat spleen tissue lysate(from left to right), using Mouse Hck Antibody (Center)(Cat. #AP20682c). AP20682c was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
  • WB - Mouse Hck Antibody (Center) AP20682c
    Western blot analysis of lysate from mouse thymus tissue lysate, using Mouse Hck Antibody (Center)(Cat. #AP20682c). AP20682c was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P08103
Other Accession P50545
Reactivity Mouse
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 59129 Da
Additional Information
Gene ID 15162
Other Names Tyrosine-protein kinase HCK, B-cell/myeloid kinase, BMK, Hematopoietic cell kinase, Hemopoietic cell kinase, p56-HCK/p59-HCK, Hck
Target/Specificity This Mouse Hck antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 239-272 amino acids from the Central region of human Mouse Hck.
Dilution WB~~1:500-1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Hck Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Hck
Function Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation of immunoglobulin gamma Fc region receptors. Phosphorylates ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1 and WAS (By similarity).
Cellular Location Cytoplasmic vesicle, secretory vesicle. Cytoplasm, cytosol Isoform 2: Cell membrane; Lipid-anchor. Membrane, caveola; Lipid-anchor. Cell junction, focal adhesion. Cytoplasm, cytoskeleton. Golgi apparatus. Cytoplasmic vesicle. Lysosome. Nucleus Note=20% of this isoform is associated with caveolae. Localization at the cell membrane and at caveolae requires palmitoylation at Cys-3. Colocalizes with the actin cytoskeleton at focal adhesions (By similarity).
Tissue Location Expressed predominantly in cells of the myeloid and B-lymphoid lineages
Research Areas
Citations (0)

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Background

Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation of immunoglobulin gamma Fc region receptors. Phosphorylates ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1 and WAS (By similarity).

References

Klemsz M.J.,et al.Nucleic Acids Res. 15:9600-9600(1987).
Holtzman D.A.,et al.Proc. Natl. Acad. Sci. U.S.A. 84:8325-8329(1987).
Carninci P.,et al.Science 309:1559-1563(2005).
Lock P.,et al.Mol. Cell. Biol. 11:4363-4370(1991).
Lowell C.A.,et al.Genes Dev. 8:387-398(1994).

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$ 295.00
$ 99.00
Cat# AP20682c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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