- CITATIONS: 1
|Application ||WB, IHC-P, E|
|Calculated MW||51444 Da|
|Antigen Region||30-59 aa|
|Other Names||Growth/differentiation factor 9, GDF-9, GDF9|
|Target/Specificity||This GDF9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-59 amino acids from the N-terminal region of human GDF9.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||GDF9 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Required for ovarian folliculogenesis. Promotes primordial follicle development. Stimulates granulosa cell proliferation. Promotes cell transition from G0/G1 to S and G2/M phases, through an increase of CCND1 and CCNE1 expression, and RB1 phosphorylation. It regulates STAR expression and cAMP-dependent progesterone release in granulosa and thecal cells. Attenuates the suppressive effects of activin A on STAR expression and progesterone production by increasing the expression of inhibin B. It suppresses FST and FSTL3 production in granulosa-lutein cells.|
|Tissue Location||Expressed in ovarian granulosa cells. Present in oocytes of primary follicles (at protein level)|
Provided below are standard protocols that you may find useful for product applications.
GDF9 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues.Growth factors synthesized by ovarian somatic cells directly affect oocyte growth and function. GDF9 is expressed in oocytes and is thought to be required for ovarian folliculogenesis.
Liao, W.X., et al., J. Biol. Chem. 278(6):3713-3719 (2003).
Vitt, U.A., et al., Biol. Reprod. 67(2):473-480 (2002).
Aaltonen, J., et al., J. Clin. Endocrinol. Metab. 84(8):2744-2750 (1999).
Dong, J., et al., Nature 383(6600):531-535 (1996).
McGrath, S.A., et al., Mol. Endocrinol. 9(1):131-136 (1995).
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