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>   home   >   Products   >   Primary Antibodies   >   Crown Antibodies   >   Histone H2A.X (Ser139) Antibody   

Histone H2A.X (Ser139) Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - Histone H2A.X (Ser139) Antibody AP20702b
    Western blot analysis of lysates from 293, K562 cell line (from left to right), using Histone H2A. X (Ser139)(Cat. #AP20702b). AP20702b was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
  • SPECIFICATION
  • CITATIONS
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  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P16104
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 15145 Da
Additional Information
Gene ID 3014
Other Names Histone H2AX, H2a/x, Histone H2AX, H2AFX, H2AX
Target/Specificity This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 134-163 amino acids from human.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHistone H2A.X (Ser139) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name H2AFX
Synonyms H2AX
Function Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C- terminal phosphorylation.
Cellular Location Nucleus Chromosome
Research Areas
Citations (0)

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Background

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C- terminal phosphorylation.

References

Mannironi C.,et al.Nucleic Acids Res. 17:9113-9126(1989).
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Rogakou E.P.,et al.J. Biol. Chem. 273:5858-5868(1998).
Rogakou E.P.,et al.J. Cell Biol. 146:905-916(1999).
Paull T.T.,et al.Curr. Biol. 10:886-895(2000).

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$ 295.00
$ 99.00
Cat# AP20702b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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