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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   MAD2L1 Antibody (C-term)   

MAD2L1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - MAD2L1 Antibody (C-term) AP20713c
    Western blot analysis of lysate from HT-29 cell line, using MAD2L1 Antibody (C-term)(Cat. #AP20713c). AP20713c was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q13257
Other Accession Q9Z1B5
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 23510 Da
Additional Information
Gene ID 4085
Other Names Mitotic spindle assembly checkpoint protein MAD2A, HsMAD2, Mitotic arrest deficient 2-like protein 1, MAD2-like protein 1, MAD2L1, MAD2
Target/Specificity This MAD2L1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 159-192 amino acids from the C-terminal region of human MAD2L1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMAD2L1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MAD2L1
Synonyms MAD2
Function Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore- spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate.
Cellular Location Nucleus. Chromosome, centromere, kinetochore. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole Note=Recruited by MAD1L1 to unattached kinetochores (Probable) Recruited to the nuclear pore complex by TPR during interphase Recruited to kinetochores in late prometaphase after BUB1, CENPF, BUB1B and CENPE. Kinetochore association requires the presence of NEK2. Kinetochore association is repressed by UBD.
Research Areas
Citations (0)

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Background

Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore- spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate.

References

Li Y.,et al.Science 274:246-248(1996).
Gemma A.,et al.Lung Cancer 32:289-295(2001).
Jin D.-Y.,et al.Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases.
Klebert S.,et al.Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases.
Nobori T.,et al.Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases.

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$ 295.00
$ 99.00
Cat# AP20713c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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