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STOM Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - STOM Antibody (N-term) AP20729a
    Western blot analysis of lysates from HepG2, Hela cell line and huamn lung tissue lysate(from left to right), using STOM Antibody (N-term)(Cat. #AP20729a). AP20729a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P27105
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 31731 Da
Additional Information
Gene ID 2040
Other Names Erythrocyte band 7 integral membrane protein, Protein 72b, Stomatin, STOM, BND7, EPB72
Target/Specificity This STOM antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 2-36 amino acids from the N-terminal region of human STOM.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSTOM Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name STOM
Synonyms BND7, EPB72
Function Regulates ion channel activity and transmembrane ion transport. Regulates ASIC2 and ASIC3 channel activity.
Cellular Location Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Cell membrane; Lipid-anchor; Cytoplasmic side. Membrane raft Melanosome. Cytoplasmic vesicle {ECO:0000250|UniProtKB:P54116}. Note=Localizes to juxtanuclear structure probably derived from the Golgi apparatus (PubMed:9243190). Colocalizes with cortical actin microfilaments at small plasma membrane protrusions (PubMed:9243190). Associates with alpha-granular lipid rafts (PubMed:12130500). Translocates from the alpha-granular lipid rafts to the cell membrane on thrombin activation and selectively enriched in released microvesicles (PubMed:12130500). Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545).
Tissue Location Detected in erythrocytes (at protein level). Widely expressed.
Research Areas
Citations (0)

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Background

Thought to regulate cation conductance. May regulate ASIC2 and ASIC3 gating (By similarity).

References

Hiebl-Dirschmied C.M.,et al.Biochim. Biophys. Acta 1090:123-124(1991).
Stewart G.W.,et al.Blood 79:1593-1601(1992).
Unfried I.,et al.Genomics 30:521-528(1995).
Gallagher P.G.,et al.J. Biol. Chem. 270:26358-26363(1995).
Halleck A.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.

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$ 295.00
$ 99.00
Cat# AP20729a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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