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ALDH4A1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - ALDH4A1 Antibody (C-term) AP20738c
    Western blot analysis of lysates from HepG2 cell line and human liver tissue lysate(from left to right), using ALDH4A1 Antibody (C-term)(Cat. #AP20738c). AP20738c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P30038
Other Accession Q8CHT0, Q7SY23, A7YWE4
Reactivity Human
Predicted Bovine, Zebrafish, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 61719 Da
Additional Information
Gene ID 8659
Other Names Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial, P5C dehydrogenase, Aldehyde dehydrogenase family 4 member A1, L-glutamate gamma-semialdehyde dehydrogenase, ALDH4A1, ALDH4, P5CDH
Target/Specificity This ALDH4A1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 522-556 amino acids from the C-terminal region of human ALDH4A1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsALDH4A1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ALDH4A1
Synonyms ALDH4, P5CDH
Function Irreversible conversion of delta-1-pyrroline-5- carboxylate (P5C), derived either from proline or ornithine, to glutamate. This is a necessary step in the pathway interconnecting the urea and tricarboxylic acid cycles. The preferred substrate is glutamic gamma-semialdehyde, other substrates include succinic, glutaric and adipic semialdehydes.
Cellular Location Mitochondrion matrix.
Tissue Location Highest expression is found in liver followed by skeletal muscle, kidney, heart, brain, placenta, lung and pancreas
Research Areas
Citations (0)

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Background

Irreversible conversion of delta-1-pyrroline-5- carboxylate (P5C), derived either from proline or ornithine, to glutamate. This is a necessary step in the pathway interconnecting the urea and tricarboxylic acid cycles. The preferred substrate is glutamic gamma-semialdehyde, other substrates include succinic, glutaric and adipic semialdehydes.

References

Hu C.-A.,et al.J. Biol. Chem. 271:9795-9800(1996).
Stagos D.,et al.Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Suzuki Y.,et al.Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
Gregory S.G.,et al.Nature 441:315-321(2006).

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$ 295.00
$ 99.00
Cat# AP20738c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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