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NDUFS3 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - NDUFS3 Antibody (C-term) AP20739c
    Western blot analysis of lysate from THP-1 cell line, using NDUFS3 Antibody (C-term)(Cat. #AP20739c). AP20739c was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
  • WB - NDUFS3 Antibody (C-term) AP20739c
    Western blot analysis of lysates from Hela, HepG2, mouse NIH/3T3 cell line, human brain and mouse brain tissue lysate(from left to right), using NDUFS3 Antibody (C-term)(Cat. #AP20739c). AP20739c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O75489
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 30242 Da
Additional Information
Gene ID 4722
Other Names NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial, Complex I-30kD, CI-30kD, NADH-ubiquinone oxidoreductase 30 kDa subunit, NDUFS3
Target/Specificity This NDUFS3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 251-284 amino acids from the C-terminal region of human NDUFS3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNDUFS3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NDUFS3
Function Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).
Cellular Location Mitochondrion inner membrane.
Research Areas
Citations (0)

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Background

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).

References

Loeffen J.,et al.Biochem. Biophys. Res. Commun. 247:751-758(1998).
Procaccio V.,et al.Mamm. Genome 11:808-810(2000).
Hu R.-M.,et al.Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

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$ 295.00
$ 99.00
Cat# AP20739c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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