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ZNRF2 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ZNRF2 Antibody (N-term) AP20753a
    Western blot analysis of lysate from Hela cell line, using ZNRF2 Antibody (N-term)(Cat. #AP20753a). AP20753a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q8NHG8
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 24115 Da
Additional Information
Gene ID 223082
Other Names E3 ubiquitin-protein ligase ZNRF2, 632-, Protein Ells2, RING finger protein 202, Zinc/RING finger protein 2, ZNRF2, RNF202
Target/Specificity This ZNRF2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 2-36 amino acids from the N-terminal region of human ZNRF2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsZNRF2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ZNRF2
Synonyms RNF202
Function May play a role in the establishment and maintenance of neuronal transmission and plasticity via its ubiquitin ligase activity. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.
Cellular Location Endosome membrane; Peripheral membrane protein. Lysosome membrane; Peripheral membrane protein. Cell junction, synapse, presynaptic cell membrane; Peripheral membrane protein. Note=Present in presynaptic plasma membranes in neurons
Tissue Location Highly expressed in the brain, with higher expression during development than in adult. Expressed also in mammary glands, testis, colon and kidney
Research Areas
Citations (0)

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Background

May play a role in the establishment and maintenance of neuronal transmission and plasticity via its ubiquitin ligase activity. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.

References

Araki T.,et al.J. Neurosci. 23:9385-9394(2003).
Guo J.H.,et al.Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases.
Olsen J.V.,et al.Cell 127:635-648(2006).
Plans V.,et al.J. Cell. Biochem. 97:572-582(2006).
Cantin G.T.,et al.J. Proteome Res. 7:1346-1351(2008).

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$ 295.00
$ 99.00
Cat# AP20753a
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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