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PRPF6 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PRPF6 Antibody (N-term) AP20777a
    All lanes : Anti-PRPF6 Antibody (N-term) at 1:6000 dilution Lane 1: HCT116 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Jurka twhole cell lysate Lane 4: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 107 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O94906
Other Accession A1A5S1, Q91YR7, Q2KJJ0
Reactivity Human, Mouse
Predicted Bovine, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 106925 Da
Additional Information
Gene ID 24148
Other Names Pre-mRNA-processing factor 6, Androgen receptor N-terminal domain-transactivating protein 1, ANT-1, PRP6 homolog, U5 snRNP-associated 102 kDa protein, U5-102 kDa protein, PRPF6, C20orf14
Target/Specificity This PRPF6 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 196-229 amino acids from the N-terminal region of human PRPF6.
Dilution WB~~1:6000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPRPF6 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PRPF6
Synonyms C20orf14
Function Involved in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex, one of the building blocks of the spliceosome (PubMed:28781166, PubMed:21549338). Enhances dihydrotestosterone- induced transactivation activity of AR, as well as dexamethasone- induced transactivation activity of NR3C1, but does not affect estrogen-induced transactivation.
Cellular Location Nucleus, nucleoplasm. Nucleus speckle. Note=Localized in splicing speckles.
Tissue Location Widely expressed..
Research Areas
Citations (0)
citation

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Background

Involved in pre-mRNA splicing as component of the U4/U6- U5 tri-snRNP complex, one of the building blocks of the spliceosome. Enhances dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but does not affect estrogen-induced transactivation.

References

Nishikimi A.,et al.Biochim. Biophys. Acta 1435:147-152(1999).
Makarov E.M.,et al.J. Mol. Biol. 298:567-575(2000).
Yuryev A.,et al.Mol. Cell. Biol. 20:4870-4878(2000).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Deloukas P.,et al.Nature 414:865-871(2001).

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$ 182.50
$ 70.00
Cat# AP20777a
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