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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   VAC14 Antibody (C-term)   

VAC14 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - VAC14 Antibody (C-term) AP20783c
    Western blot analysis of lysate from rat spleen tissue lysate, using VAC14 Antibody (C-term)(Cat. #AP20783c). AP20783c was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q08AM6
Other Accession Q80W92, A2VE70
Reactivity Rat
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 87973 Da
Additional Information
Gene ID 55697
Other Names Protein VAC14 homolog, Tax1-binding protein 2, VAC14, TAX1BP2, TRX
Target/Specificity This VAC14 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 769-802 amino acids from the C-terminal region of human VAC14.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsVAC14 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VAC14
Synonyms TAX1BP2, TRX
Function The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Acts as a positive activator of PIKfyve kinase activity. Also required to maintain normal levels of phosphatidylinositol 3-phosphate (PtdIns(3)P) and phosphatidylinositol 5-phosphate (PtdIns(5)P). Plays a role in the biogenesis of endosome carrier vesicles (ECV) / multivesicular bodies (MVB) transport intermediates from early endosomes.
Cellular Location Endosome membrane Microsome membrane. Note=Mainly associated with membranes of the late endocytic pathway
Tissue Location Ubiquitously expressed.
Research Areas
Citations (0)

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Background

The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Acts as a positive activator of PIKfyve kinase activity. Also required to maintain normal levels of phosphatidylinositol 3-phosphate (PtdIns(3)P) and phosphatidylinositol 5-phosphate (PtdIns(5)P). Plays a role in the biogenesis of endosome carrier vesicles (ECV) / multivesicular bodies (MVB) transport intermediates from early endosomes.

References

Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mireskandari A.,et al.Biochim. Biophys. Acta 1306:9-13(1996).
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Sbrissa D.,et al.Mol. Cell. Biol. 24:10437-10447(2004).
Lemaire J.F.,et al.FEBS Lett. 580:6948-6954(2006).

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$ 295.00
$ 99.00
Cat# AP20783c
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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