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SLC2A4 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - SLC2A4 Antibody (C-term) AP20792a
    Western blot analysis of lysate from human heart tissue, using SLC2A4 Antibody (C-term)(Cat. #AP20792a). AP20792a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P14672
Other Accession P19357, P14142
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 54787 Da
Additional Information
Gene ID 6517
Other Names Solute carrier family 2, facilitated glucose transporter member 4, Glucose transporter type 4, insulin-responsive, GLUT-4, SLC2A4, GLUT4
Target/Specificity This SLC2A4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 495-529 amino acids from the C-terminal region of human SLC2A4.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSLC2A4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SLC2A4 (HGNC:11009)
Function Insulin-regulated facilitative glucose transporter, which plays a key role in removal of glucose from circulation. Response to insulin is regulated by its intracellular localization: in the absence of insulin, it is efficiently retained intracellularly within storage compartments in muscle and fat cells. Upon insulin stimulation, translocates from these compartments to the cell surface where it transports glucose from the extracellular milieu into the cell.
Cellular Location Cell membrane {ECO:0000250|UniProtKB:P14142}; Multi-pass membrane protein {ECO:0000250|UniProtKB:P14142} Endomembrane system; Multi-pass membrane protein. Cytoplasm, perinuclear region {ECO:0000250|UniProtKB:P14142}. Note=Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized (PubMed:8300557). The dileucine internalization motif is critical for intracellular sequestration (PubMed:8300557). Insulin stimulation induces translocation to the cell membrane (By similarity) {ECO:0000250|UniProtKB:P14142, ECO:0000269|PubMed:8300557}
Tissue Location Skeletal and cardiac muscles; brown and white fat.
Research Areas
Citations (0)
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Background

Insulin-regulated facilitative glucose transporter.

References

Fukumoto H.,et al.J. Biol. Chem. 264:7776-7779(1989).
Buse J.B.,et al.Diabetes 41:1436-1445(1992).
Chiaramonte R.,et al.Gene 130:307-308(1993).
Verhey K.J.,et al.J. Biol. Chem. 269:2353-2356(1994).
Lalioti V.S.,et al.J. Biol. Chem. 277:19783-19791(2002).

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$ 365.00
$ 140.00
Cat# AP20792a
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