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MUC4(Mucin-4 alpha chain) Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - MUC4(Mucin-4 alpha chain) Antibody (N-term) AP20801a
    Western blot analysis of lysates from Hela, MCF-7 cell line (from left to right), using MUC4(Mucin-4 alpha chain) Antibody (N-term)(Cat. #AP20801a). AP20801a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q99102
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 231518 Da
Additional Information
Gene ID 4585
Other Names Mucin-4, MUC-4, Ascites sialoglycoprotein, ASGP, Pancreatic adenocarcinoma mucin, Testis mucin, Tracheobronchial mucin, Mucin-4 alpha chain, Ascites sialoglycoprotein 1, ASGP-1, Mucin-4 beta chain, Ascites sialoglycoprotein 2, ASGP-2, MUC4
Target/Specificity This MUC4(Mucin-4 alpha chain) antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 529-560 amino acids from the N-terminal region of human MUC4(Mucin-4 alpha chain).
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMUC4(Mucin-4 alpha chain) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MUC4
Function May play a role in tumor progression. Ability to promote tumor growth may be mainly due to repression of apoptosis as opposed to proliferation. Has anti-adhesive properties. Seems to alter cellular behavior through both anti-adhesive effects on cell-cell and cell-extracellular matrix interactions and in its ability to act as an intramembrane ligand for ERBB2. Plays an important role in cell proliferation and differentiation of epithelial cells by inducing specific phosphorylation of ERBB2. The MUC4-ERBB2 complex causes site-specific phosphorylation of the ERBB2 'Tyr-1248'. In polarized epithelilal cells segragates ERBB2 and other ERBB receptors and prevents ERBB2 from acting as a coreceptor. The interaction with ERBB2 leads to enhanced expression of CDKN1B. The formation of a MUC4-ERBB2-ERBB3-NRG1 complex leads to down-regulation of CDKN1B, resulting in repression of apoptosis and stimulation of proliferation.
Cellular Location Membrane; Single-pass membrane protein. Secreted Note=Isoforms lacking the Cys-rich region, EGF-like domains and transmembrane region are secreted. Secretion occurs by splicing or proteolytic processing Mucin-4 alpha chain: Secreted. Isoform 11: Secreted. Isoform 17: Cell membrane; Single-pass membrane protein
Tissue Location Expressed in the thymus, thyroid, lung, trachea, esophagus, stomach, small intestine, colon, testis, prostate, ovary, uterus, placenta, and mammary and salivary glands. Expressed in carcinomas arising from some of these epithelia, such as lung cancers, squamous cell carcinomas of the upper aerodigestive tract, mammary carcinomas, biliary tract, colon, and cervix cancers. Minimally or not expressed in the normal pancreas or chronic pancreatitis, but is highly expressed in pancreatic tumors and pancreatic tumor cell lines
Research Areas
Citations (0)

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Background

May play a role in tumor progression. Ability to promote tumor growth may be mainly due to repression of apoptosis as opposed to proliferation. Has anti-adhesive properties. Seems to alter cellular behavior through both anti-adhesive effects on cell-cell and cell-extracellular matrix interactions and in its ability to act as an intramembrane ligand for ERBB2. Plays an important role in cell proliferation and differentiation of epithelial cells by inducing specific phosphorylation of ERBB2. The MUC4-ERBB2 complex causes site-specific phosphorylation of the ERBB2 'Tyr-1248'. In polarized epithelilal cells segragates ERBB2 and other ERBB receptors and prevents ERBB2 from acting as a coreceptor. The interaction with ERBB2 leads to enhanced expression of CDKN1B. The formation of a MUC4-ERBB2-ERBB3-NRG1 complex leads to down-regulation of CDKN1B, resulting in repression of apoptosis and stimulation of proliferation.

References

Moniaux N.,et al.Eur. J. Biochem. 267:4536-4544(2000).
Choudhury A.,et al.J. Biochem. 128:233-243(2000).
Desseyn J.-L.,et al.Eur. J. Biochem. 269:3150-3159(2002).
Moniaux N.,et al.Biochem. J. 338:325-333(1999).
Escande F.,et al.Eur. J. Biochem. 269:3637-3644(2002).

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$ 295.00
$ 99.00
Cat# AP20801a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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