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MOUSE Brd7 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - MOUSE Brd7 Antibody (Center) AP20823c
    All lanes : Anti-Brd7 Antibody (Center) at 1:2000 dilution Lane 1: mouse heart lysate Lane 2: mouse kidney lysate Lane 3: mouse lung lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 74 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - MOUSE Brd7 Antibody (Center) AP20823c
    Western blot analysis of lysate from mouse lung tissue lysate, using MOUSE Brd7 Antibody (Center)(Cat. #AP20823c). AP20823c was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35ug.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O88665
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 74000 Da
Additional Information
Gene ID 26992
Other Names Bromodomain-containing protein 7, 75 kDa bromodomain protein, Brd7, Bp75
Target/Specificity This MOUSE Brd7 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 387-418 amino acids from the Central region of human MOUSE Brd7.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMOUSE Brd7 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Brd7
Synonyms Bp75
Function Acts both as coactivator and as corepressor. May play a role in chromatin remodeling. Transcriptional corepressor that down-regulates the expression of target genes. Binds to target promoters, leading to increased histone H3 acetylation at 'Lys-9' (H3K9ac). Binds to the ESR1 promoter. Recruits BRCA1 and POU2F1 to the ESR1 promoter. Coactivator for TP53-mediated activation of transcription of a set of target genes. Required for TP53-mediated cell-cycle arrest in response to oncogene activation. Promotes acetylation of TP53 at 'Lys-382', and thereby promotes efficient recruitment of TP53 to target promoters. Inhibits cell cycle progression from G1 to S phase (By similarity). Activator of the Wnt signaling pathway in a DVL1-dependent manner by negatively regulating the GSK3B phosphotransferase activity. Induces dephosphorylation of GSK3B at 'Tyr-216'. Down-regulates TRIM24- mediated activation of transcriptional activation by AR.
Cellular Location Nucleus
Tissue Location Ubiquitous..
Citations (0)

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Background

Acts both as coactivator and as corepressor. May play a role in chromatin remodeling. Transcriptional corepressor that down-regulates the expression of target genes. Binds to target promoters, leading to increased histone H3 acetylation at 'Lys-9' (H3K9ac). Binds to the ESR1 promoter. Recruits BRCA1 and POU2F1 to the ESR1 promoter. Coactivator for TP53-mediated activation of transcription of a set of target genes. Required for TP53-mediated cell-cycle arrest in response to oncogene activation. Promotes acetylation of TP53 at 'Lys-382', and thereby promotes efficient recruitment of TP53 to target promoters. Inhibits cell cycle progression from G1 to S phase (By similarity). Activator of the Wnt signaling pathway in a DVL1-dependent manner by negatively regulating the GSK3B phosphotransferase activity. Induces dephosphorylation of GSK3B at 'Tyr-216'. Down-regulates TRIM24- mediated activation of transcriptional activation by AR.

References

Cuppen E.,et al.FEBS Lett. 459:291-298(1999).
Carninci P.,et al.Science 309:1559-1563(2005).
Kim S.,et al.Cancer Res. 63:4792-4795(2003).
Kaeser M.D.,et al.J. Biol. Chem. 283:32254-32263(2008).
Kikuchi M.,et al.Biochim. Biophys. Acta 1793:1828-1836(2009).

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$ 295.00
$ 99.00
Cat# AP20823c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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