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Mouse Nkx2-5 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - Mouse Nkx2-5 Antibody (Center) AP20850c
    All lanes : Anti-Mouse Nkx2-5 Antibody (Center) at 1:2000 dilution Lane 1: human heart lysate Lane 2: mouse heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 34 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - Mouse Nkx2-5 Antibody (Center) AP20850c
    Anti-Mouse Nkx2-5 Antibody (Center) at 1:2000 dilution + CCRF-CEM whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 34 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - Mouse Nkx2-5 Antibody (Center) AP20850c
    Western blot analysis of lysates from mouse stomach, rat heart tissue (from left to right), using Mouse Nkx2-5 Antibody (Center)(Cat. #AP20850c). AP20850c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P42582
Other Accession O35767
Reactivity Mouse, Rat, Hamster
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 34163 Da
Additional Information
Gene ID 18091
Other Names Homeobox protein Nkx-25, Cardiac-specific homeobox, Homeobox protein CSX, Homeobox protein NK-2 homolog E, Nkx2-5, Csx, Nkx-25, Nkx2e
Target/Specificity This Mouse Nkx2-5 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 192-225 amino acids from the Central region of human Mouse Nkx2-5.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Nkx2-5 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Nkx2-5
Synonyms Csx, Nkx-2.5, Nkx2e
Function Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4. It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B. Together with PBX1, it is required for spleen development through a mechanism that involves CDKN2B repression.
Cellular Location Nucleus.
Tissue Location Predominantly in the adult and embryonic heart, and to a lesser extent in lingual muscle, spleen and stomach
Research Areas
Citations (0)

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Background

Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4. It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B. Together with PBX1, it is required for spleen development through a mechanism that involves CDKN2B repression.

References

Lints T.J.,et al.Development 119:419-431(1993).
Lints T.J.,et al.Development 119:969-969(1993).
Searcy R.D.,et al.Development 125:4461-4470(1998).
Komuro I.,et al.Proc. Natl. Acad. Sci. U.S.A. 90:8145-8149(1993).
Kim Y.H.,et al.J. Biol. Chem. 273:25875-25879(1998).

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$ 295.00
$ 99.00
Cat# AP20850c
Size:
Quantity:
(40 western blots)
Availability: in Stock
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