- CITATIONS: 1
|Application ||WB, E|
|Calculated MW||41146 Da|
|Other Names||Lysosomal-associated transmembrane protein 4B, Lysosome-associated transmembrane protein 4-beta, LAPTM4B|
|Target/Specificity||This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 80-114 amino acids from human.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||LAPTM4B-3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Required for optimal lysosomal function (PubMed:21224396). Blocks EGF-stimulated EGFR intraluminal sorting and degradation. Conversely by binding with the phosphatidylinositol 4,5-bisphosphate, regulates its PIP5K1C interaction, inhibits HGS ubiquitination and relieves LAPTM4B inhibition of EGFR degradation (PubMed:25588945). Recruits SLC3A2 and SLC7A5 (the Leu transporter) to the lysosome, promoting entry of leucine and other essential amino acid (EAA) into the lysosome, stimulating activation of proton-transporting vacuolar (V)-ATPase protein pump (V-ATPase) and hence mTORC1 activation (PubMed:25998567). Plays a role as negative regulator of TGFB1 production in regulatory T cells (PubMed:26126825). Binds ceramide and facilitates its exit from late endosome in order to control cell death pathways (PubMed:26280656).|
|Cellular Location||Endomembrane system; Multi-pass membrane protein. Late endosome membrane. Cell membrane. Cell projection. Lysosome membrane Endosome membrane. Endosome, multivesicular body membrane. Endosome, multivesicular body lumen|
Provided below are standard protocols that you may find useful for product applications.
Shao G.-Z.,et al.Oncogene 22:5060-5069(2003).
Hogue D.L.,et al.Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases.
Otsuki T.,et al.DNA Res. 12:117-126(2005).
Wiemann S.,et al.Genome Res. 11:422-435(2001).
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