|Application ||WB, IHC-P, E|
|Calculated MW||47773 Da|
|Other Names||Sonic hedgehog protein, SHH, HHG-1, Sonic hedgehog protein N-product, Sonic hedgehog protein 19 kDa product, Sonic hedgehog protein C-product, Sonic hedgehog protein 27 kDa product, Shh, Hhg1|
|Target/Specificity||This (Mouse) Shh antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 397-431 amino acids from the C-terminal region of Mouse Shh.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||(Mouse) Shh Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Sonic hedgehog protein: The C-terminal part of the sonic hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity (PubMed:8824192, PubMed:7891723). Both activities result in the cleavage of the full-length protein into two parts (ShhN and ShhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated ShhN (PubMed:8824192). Both activities occur in the reticulum endoplasmic (PubMed:21357747). Once cleaved, ShhC is degraded in the endoplasmic reticulum (PubMed:21357747).|
|Cellular Location||Sonic hedgehog protein N-product: Cell membrane; Lipid-anchor. Note=The dual-lipidated sonic hedgehog protein N-product (ShhNp) is firmly tethered to the cell membrane where it forms multimers (PubMed:24522195). Further solubilization and release from the cell surface seem to be achieved through different mechanisms, including the interaction with DISP1 and SCUBE2, movement by lipoprotein particles, transport by cellular extensions called cytonemes or by the proteolytic removal of both terminal lipidated peptides|
|Tissue Location||Expressed in a number of embryonic tissues including the notochord, ventral neural tube, floor plate, lung bud, zone of polarizing activity and posterior distal mesenchyme of limbs. In the adult, expressed in lung and neural retina|
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Provided below are standard protocols that you may find useful for product applications.
Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction (By similarity).
Echelard Y.,et al.Cell 75:1417-1430(1993).
McMahon A.P.,et al.Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases.
Chang D.T.,et al.Development 120:3339-3353(1994).
Carninci P.,et al.Science 309:1559-1563(2005).
Roelink H.,et al.Cell 81:445-455(1995).
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