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>   home   >   Products   >   Primary Antibodies   >   (Mouse) Zcchc11 Antibody (C-term)   

(Mouse) Zcchc11 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - (Mouse) Zcchc11 Antibody (C-term) AP20889c
    Western blot analysis of lysates from mouse spleen, mouse thymus tissue lysate (from left to right), using Zcchc11 Antibody (C-term)(Cat. #AP20889c). AP20889c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession B2RX14
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 184650 Da
Additional Information
Gene ID 230594
Other Names Terminal uridylyltransferase 4, TUTase 4, Zinc finger CCHC domain-containing protein 11, Zcchc11, Kiaa0191, Tut4
Target/Specificity This (Mouse) Zcchc11 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1568-1600 amino acids from the C-terminal region of (Mouse) Zcchc11.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions(Mouse) Zcchc11 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Zcchc11
Synonyms Kiaa0191, Tut4
Function Uridylyltransferase that mediates the terminal uridylation of mRNAs with short (less than 25 nucleotides) poly(A) tails, hence facilitating global mRNA decay. Involved in microRNA (miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets. Also acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of some miRNA precursors, including that of let-7 (pre-let-7), miR107, miR-143 and miR-200c. Uridylated miRNAs are not processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem cell pluripotency (PubMed:19703396). Does not bind RNA directly, but recruited to RNA targets by RNA- binding protein LIN28A. Also catalyzes the 3' uridylation of miR- 26A, a miRNA that targets IL6 transcript. This abrogates the silencing of IL6 transcript, hence promoting cytokine expression (PubMed:19701194). May also suppress Toll-like receptor-induced NF-kappa-B activation via binding to T2BP. Does not play a role in replication-dependent histone mRNA degradation. Due to functional redundancy between ZCCHC6 and ZCCHC11, the identification of the specific role of each of these proteins is difficult.
Cellular Location Nucleus {ECO:0000250|UniProtKB:Q5TAX3}. Cytoplasm. Note=Mainly cytoplasmic (PubMed:19703396). Translocates into the cytoplasm following treatment of the cell with LPS (By similarity) {ECO:0000250|UniProtKB:Q5TAX3, ECO:0000269|PubMed:19703396}
Tissue Location Ubiquitously expressed.
Citations (0)

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Background

Uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. Catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre- let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency. Does not bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28A and LIN28B (By similarity). Also catalyzes the 3' uridylation of miR- 26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression. May also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP. Does not play a role in replication-dependent histone mRNA degradation (By similarity).

References

Church D.M.,et al.PLoS Biol. 7:E1000112-E1000112(2009).
Okazaki N.,et al.Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
Carninci P.,et al.Science 309:1559-1563(2005).
Trost M.,et al.Immunity 30:143-154(2009).
Jones M.R.,et al.Nat. Cell Biol. 11:1157-1163(2009).

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$ 295.00
$ 99.00
Cat# AP20889c
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(40 western blots)
Availability: In Stock
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