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EPOR Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • FC - EPOR Antibody (C-term) AP20930c
    Overlay histogram showing K562 cells stained with AP20930C(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP20930C, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • FC - EPOR Antibody (C-term) AP20930c
    Overlay histogram showing K562 cells stained with AP20930C(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP20930C, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • FC - EPOR Antibody (C-term) AP20930c
    Overlay histogram showing K562 cells stained with AP20930C(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP20930C, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • WB - EPOR Antibody (C-term) AP20930c
    All lanes : Anti-EPOR Antibody (C-term) at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: HL-60 whole cell lysate Lane 4: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 55 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - EPOR Antibody (C-term) AP20930c
    All lanes : Anti-EPOR Antibody (C-term) at 1:2000 dilution Lane 1: H. kidney whole lysate Lane 2: H. placenta whole lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 55 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - EPOR Antibody (C-term) AP20930c
    Western blot analysis of lysates from K562, Jurkat, HL-60, HepG2 cell line, human kidney, human placenta tissue(from left to right), using EPOR Antibody (C-term)(Cat. #AP20930c). AP20930c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, WB, E
Primary Accession P19235
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 55065 Da
Additional Information
Gene ID 2057
Other Names Erythropoietin receptor, EPO-R, EPOR
Target/Specificity This EPOR antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 470-504 amino acids from the C-terminal region of human EPOR.
Dilution FC~~1:25
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEPOR Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EPOR
Function Receptor for erythropoietin. Mediates erythropoietin- induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase.
Cellular Location Cell membrane; Single-pass type I membrane protein
Tissue Location Erythroid cells and erythroid progenitor cells. Isoform EPOR-F is the most abundant form in EPO-dependent erythroleukemia cells and in late-stage erythroid progenitors Isoform EPOR-S and isoform EPOR-T are the predominant forms in bone marrow. Isoform EPOR-T is the most abundant from in early- stage erythroid progenitor cells
Research Areas
Citations (0)

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Background

Receptor for erythropoietin. Mediates erythropoietin- induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase.

References

Winkelmann J.C.,et al.Blood 76:24-30(1990).
Jones S.S.,et al.Blood 76:31-35(1990).
Noguchi C.T.,et al.Blood 78:2548-2556(1991).
Ehrenman K.,et al.Exp. Hematol. 19:973-977(1991).
Nakamura Y.,et al.Science 257:1138-1141(1992).

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$ 295.00
$ 99.00
Cat# AP20930c
Size:
Quantity:
(40 western blots)
Availability: in Stock
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