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PPP2R5B Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IF - PPP2R5B Antibody (C-term) AP20958c
    Fluorescent image of SH-SY5Y cells stained with PPP2R5B Antibody (C-term)(Cat#AP20958c). AP20958c was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
  • WB - PPP2R5B Antibody (C-term) AP20958c
    Western blot analysis of lysate from SK-N-MC cell line, using PPP2R5B Antibody (C-term)(Cat. #AP20958c). AP20958c was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession Q15173
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 57393 Da
Additional Information
Gene ID 5526
Other Names Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform, PP2A B subunit isoform B'-beta, PP2A B subunit isoform B56-beta, PP2A B subunit isoform PR61-beta, PP2A B subunit isoform R5-beta, PPP2R5B
Target/Specificity This PPP2R5B antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 466-500 amino acids from the C-terminal region of human PPP2R5B.
Dilution IF~~1:25
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPPP2R5B Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PPP2R5B
Function As the regulatory component of the serine/threonine- protein phosphatase 2A (PP2A) holoenzyme, modulates substrate specificity, subcellular localization, and responsiveness to phosphorylation. The phosphorylated form mediates the interaction between PP2A and AKT1, leading to AKT1 dephosphorylation.
Cellular Location Cytoplasm.
Tissue Location Highest expression in brain.
Research Areas
Citations (0)

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Background

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. The phosphorylated form mediates the interaction between AKT1 and PP2A phosphatase leading to dephosphorylation of AKT1 on the 'Thr-308' and 'Ser-373' residues.

References

McCright B.,et al.J. Biol. Chem. 270:26123-26128(1995).
Zolnierowicz S.,et al.Biochem. J. 317:187-194(1996).
McCright B.,et al.J. Biol. Chem. 271:22081-22089(1996).
Kitajima T.S.,et al.Nature 441:46-52(2006).
Rodgers J.T.,et al.Mol. Cell 41:471-479(2011).

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$ 295.00
$ 99.00
Cat# AP20958c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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