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>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   Mouse Ephb1 Antibody (Center)   

Mouse Ephb1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - Mouse Ephb1 Antibody (Center) AP20994a
    All lanes : Anti-Ephb1 Antibody (Center) at 1:1000 dilution Lane 1: mouse brain lysates Lane 2: mouse heart lysates Lane 3: rat brain lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 110 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - Mouse Ephb1 Antibody (Center) AP20994a
    Western blot analysis of lysates from A431, mouse NIH/3T3 cell line, mouse brain, rat brain tissue lysate(from left to right), using Ephb1 Antibody (Center)(Cat. #AP20994a). AP20994a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q8CBF3
Other Accession P54754, P54753, P09759, P54762, Q07494
Reactivity Human, Mouse, Rat
Predicted Chicken
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 109881 Da
Additional Information
Gene ID 270190
Other Names Ephrin type-B receptor 1, Ephb1
Target/Specificity This Mouse Ephb1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 374-409 amino acids from the Central region of Mouse Ephb1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Ephb1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Ephb1
Function Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Cognate/functional ephrin ligands for this receptor include EFNB1, EFNB2 and EFNB3. During nervous system development, regulates retinal axon guidance redirecting ipsilaterally ventrotemporal retinal ganglion cells axons at the optic chiasm midline. This probably requires repulsive interaction with EFNB2. In the adult nervous system together with EFNB3, regulates chemotaxis, proliferation and polarity of the hippocampus neural progenitors. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and synapse formation. May also regulate angiogenesis. More generally, may play a role in targeted cell migration and adhesion. Upon activation by EFNB1 and probably other ephrin-B ligands activates the MAPK/ERK and the JNK signaling cascades to regulate cell migration and adhesion respectively.
Cellular Location Cell membrane; Single-pass type I membrane protein. Early endosome membrane. Cell projection, dendrite
Tissue Location Expressed in neural stem and progenitor cells in the dentate gyrus.
Research Areas
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Background

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Cognate/functional ephrin ligands for this receptor include EFNB1, EFNB2 and EFNB3. During nervous system development, regulates retinal axon guidance redirecting ipsilaterally ventrotemporal retinal ganglion cells axons at the optic chiasm midline. This probably requires repulsive interaction with EFNB2. In the adult nervous system together with EFNB3, regulates chemotaxis, proliferation and polarity of the hippocampus neural progenitors. Beside its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and synapse formation. May also regulate angiogenesis. More generally, may play a role in targeted cell migration and adhesion. Upon activation by EFNB1 and probably other ephrin-B ligands activates the MAPK/ERK and the JNK signaling cascades to regulate cell migration and adhesion respectively.

References

Carninci P.,et al.Science 309:1559-1563(2005).
Church D.M.,et al.PLoS Biol. 7:E1000112-E1000112(2009).
Stein E.,et al.J. Biol. Chem. 273:1303-1308(1998).
Torres R.,et al.Neuron 21:1453-1463(1998).
Han D.C.,et al.J. Biol. Chem. 277:45655-45661(2002).

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$ 295.00
$ 99.00
Cat# AP20994a
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(40 western blots)
Availability: In Stock
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