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(Mouse) Smad2 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - (Mouse) Smad2 Antibody (Center) AP21006a
    Western blot analysis of lysates from Hela, mouse NIH/3T3, mouse C2C12, rat PC-12 cell line (from left to right), using Smad2 Antibody (Center)(Cat. #AP21006a). AP21006a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q62432
Other Accession P84025, P84024, Q8BUN5, P84022, P84023, O70436, Q15796, Q1W668
Reactivity Human, Mouse, Rat
Predicted Bovine, Chicken, Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 52266 Da
Additional Information
Gene ID 17126
Other Names Mothers against decapentaplegic homolog 2, MAD homolog 2, Mothers against DPP homolog 2, Mad-related protein 2, mMad2, SMAD family member 2, SMAD 2, Smad2, Smad2, Madh2, Madr2
Target/Specificity This mouse Smad2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 198-232 amino acids from the Central region of mouse Smad2.
Dilution WB~~1:500-1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions(Mouse) Smad2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Smad2
Synonyms Madh2, Madr2
Function Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator (By similarity).
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:Q15796}. Nucleus {ECO:0000250|UniProtKB:Q15796}. Note=Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1. {ECO:0000250|UniProtKB:Q15796}
Research Areas
Citations (0)

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Background

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator (By similarity).

References

Baker J.C.,et al.Genes Dev. 10:1880-1889(1996).
Devereux T.R.,et al.Carcinogenesis 18:1751-1755(1997).
Bernard D.J.,et al.Mol. Endocrinol. 18:606-623(2004).
Carninci P.,et al.Science 309:1559-1563(2005).
Weinstein M.,et al.Cytokine Growth Factor Rev. 11:49-58(2000).

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$ 295.00
$ 99.00
Cat# AP21006a
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Quantity:
(40 western blots)
Availability: In Stock
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