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(Mouse) Smad2 Antibody (Center)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q62432 |
| Other Accession | P84025, P84024, Q8BUN5, P84022, P84023, O70436, Q15796, Q1W668 |
| Reactivity | Human, Mouse, Rat |
| Predicted | Bovine, Chicken, Pig |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 52266 Da |
| Gene ID | 17126 |
|---|---|
| Other Names | Mothers against decapentaplegic homolog 2, MAD homolog 2, Mothers against DPP homolog 2, Mad-related protein 2, mMad2, SMAD family member 2, SMAD 2, Smad2, Smad2, Madh2, Madr2 |
| Target/Specificity | This mouse Smad2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 198-232 amino acids from the Central region of mouse Smad2. |
| Dilution | WB~~1:500-1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | (Mouse) Smad2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | Smad2 |
|---|---|
| Synonyms | Madh2, Madr2 |
| Function | Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. Promotes TGFB1-mediated transcription of odontoblastic differentiation genes in dental papilla cells (PubMed:33548622). Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator (By similarity). |
| Cellular Location | Cytoplasm. Nucleus. Note=Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4 or with IPO7 (PubMed:21145499, PubMed:33548622). On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1 (By similarity). Localized mainly to the nucleus in the early stages of embryo development with expression becoming evident in the cytoplasm at the blastocyst and epiblast stages (PubMed:21145499). {ECO:0000250|UniProtKB:Q15796, ECO:0000269|PubMed:21145499, ECO:0000269|PubMed:33548622} |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator (By similarity).
References
Baker J.C.,et al.Genes Dev. 10:1880-1889(1996).
Devereux T.R.,et al.Carcinogenesis 18:1751-1755(1997).
Bernard D.J.,et al.Mol. Endocrinol. 18:606-623(2004).
Carninci P.,et al.Science 309:1559-1563(2005).
Weinstein M.,et al.Cytokine Growth Factor Rev. 11:49-58(2000).
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