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TLE1 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - TLE1 Antibody (N-term) AP21035a
    Western blot analysis of lysates from HepG2, MCF-7 cell line and human brain tissue(from left to right), using TLE1 Antibody (N-term)(Cat. #AP21035a). AP21035a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q04724
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 83201 Da
Additional Information
Gene ID 7088
Other Names Transducin-like enhancer protein 1, E(Sp1) homolog, Enhancer of split groucho-like protein 1, ESG1, TLE1
Target/Specificity This TLE1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 187-221 amino acids from the N-terminal region of human TLE1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTLE1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TLE1
Function Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.
Cellular Location Nucleus. Note=Nuclear and chromatin-associated, depending on isoforms and phosphorylation status. Hyperphosphorylation decreases the affinity for nuclear components
Tissue Location In all tissues examined, mostly in brain, liver and muscle
Research Areas
Citations (0)

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Background

Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.

References

Stifani S.,et al.Nat. Genet. 2:119-127(1992).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Humphray S.J.,et al.Nature 429:369-374(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Wang J.-C.,et al.J. Biol. Chem. 275:18418-18423(2000).

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$ 295.00
$ 99.00
Cat# AP21035a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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