|Application ||IHC-P, WB, E|
|Calculated MW||40737 Da|
|Antigen Region||347-377 aa|
|Other Names||26S proteasome non-ATPase regulatory subunit 4, 26S proteasome regulatory subunit RPN10, 26S proteasome regulatory subunit S5A, Antisecretory factor 1, AF, ASF, Multiubiquitin chain-binding protein, PSMD4, MCB1|
|Target/Specificity||This PSMD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 347-377 amino acids from the C-terminal region of human PSMD4.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PSMD4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Binds and presumably selects ubiquitin-conjugates for destruction. Displays selectivity for longer polyubiquitin chains. Modulates intestinal fluid secretion.|
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Provided below are standard protocols that you may find useful for product applications.
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSDM4 one of the non-ATPase subunits of the 19S regulator lid.
Walters, K.J., et al., Biochemistry 41(6):1767-1777 (2002).
Kawahara, H., et al., EMBO J. 19(15):4144-4153 (2000).
Tanahashi, N., et al., J. Biol. Chem. 275(19):14336-14345 (2000).
Johansson, E., et al., J. Biol. Chem. 270(35):20615-20620 (1995).
Coux, O., et al., Annu. Rev. Biochem. 65, 801-847 (1996).
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