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(Mouse) Tdgf1 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - (Mouse) Tdgf1 Antibody (N-term) AP21046a
    Western blot analysis of lysate from mouse F9 cell line, using Tdgf1 Antibody (N-term)(Cat. #AP21046a). AP21046a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P51865
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 18655 Da
Additional Information
Gene ID 21667
Other Names Teratocarcinoma-derived growth factor, Cripto growth factor, Epidermal growth factor-like Cripto protein, Tdgf1, Cripto
Target/Specificity This Mouse Tdgf1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 37-70 amino acids from the N-terminal region of mouse Tdgf1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions(Mouse) Tdgf1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Cripto
Synonyms Tdgf1
Function GPI-anchored cell membrane protein involved in Nodal signaling. Cell-associated CRIPTO acts as a Nodal coreceptor in cis. Shedding of CRIPTO by Tmem8a modulates Nodal signaling by allowing soluble CRIPTO to act as a Nodal coreceptor on other cells. Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.
Cellular Location Cell membrane; Lipid-anchor, GPI-anchor. Secreted {ECO:0000250|UniProtKB:P13385}. Note=Released from the cell membrane by GPI cleavage. {ECO:0000250|UniProtKB:P13385}
Tissue Location Expressed at low level in specific organs of the adult animal such as spleen, heart, lung and brain. During gastrulation, expressed in the forming mesoderm. In later stages of the developing heart, expression is restricted to the truncus arteriosus
Research Areas
Citations (0)
citation

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Background

Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.

References

Dono R.,et al.Development 118:1157-1168(1993).
Liguori G.,et al.Mamm. Genome 7:344-348(1996).
Minchiotti G.,et al.Mech. Dev. 90:133-142(2000).
Calvanese L.,et al.J. Med. Chem. 49:7054-7062(2006).

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$ 182.50
$ 70.00
Cat# AP21046a
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