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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   SMURF1 Antibody (N-term)   

SMURF1 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - SMURF1 Antibody (N-term) AP2104a
    Western blot analysis of anti-SMURF1 Pab (Cat. #AP2104a) in mouse kidney tissue lysate (35ug/lane). SMURF1(arrow) was detected using the purified Pab.
  • IHC-P - SMURF1 Antibody (N-term) AP2104a
    Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with hSMURF1-W81 (Cat.#AP2104a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • IHC-P - SMURF1 Antibody (N-term) AP2104a
    Formalin-fixed and paraffin-embedded human Testis tissue reacted with hSMURF1-W81 (Cat.#AP2104a), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9HCE7
Other Accession Q2TAS2, A2A5Z6, Q9HAU4, A9JRZ0, Q9PUN2, Q9CUN6
Reactivity Human, Mouse
Predicted Zebrafish, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 86114 Da
Antigen Region 66-96 aa
Additional Information
Gene ID 57154
Other Names E3 ubiquitin-protein ligase SMURF1, hSMURF1, 632-, SMAD ubiquitination regulatory factor 1, SMAD-specific E3 ubiquitin-protein ligase 1, SMURF1, KIAA1625
Target/Specificity This SMURF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-96 amino acids from the N-terminal region of human SMURF1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSMURF1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms KIAA1625
Function E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA.
Cellular Location Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side
Research Areas
Citations ( 0 )


Members of the transforming growth factor-beta (TGFB) family signal through type I and type II serine/threonine-kinase receptors, which in turn activate the SMAD signaling pathway. Bone morphogenetic protein (BMP) receptors target SMAD1, SMAD5, and SMAD8, whereas receptors for activin and TGFB (e.g., ACVR1 and TGFBR1, respectively) target SMAD2 and SMAD3. Phosphorylation of these receptor-regulated SMADs induces their association with the common-partner SMAD, SMAD4. Smurf1, a HECT domain E3 ubiquitin ligase, regulates cell polarity and protrusive activity and is required to maintain the transformed morphology and motility of a tumor cell. Atypical protein kinase C-zeta (PKC2), an effector of the Cdc42/Rac1-PAR6 polarity complex, recruits Smurf1 to cellular protrusions, where it controlled the local level of RhoA. Smurf1 thus links the polarity complex to degradation of RhoA in lamellipodia and filopodia to prevent RhoA signaling during dynamic membrane movements.


Tajima, Y., et al., J. Biol. Chem. 278(12):10716-10721 (2003). Suzuki, C., et al., J. Biol. Chem. 277(42):39919-39925 (2002). Ebisawa, T., et al., J. Biol. Chem. 276(16):12477-12480 (2001). Zhu, H., et al., Nature 400(6745):687-693 (1999). Lambris, J., et al., J. Immunol. Methods 27(1):55-59 (1979).

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$ 295.00
$ 99.00
Cat# AP2104a
(40 western blots)
Availability: In Stock
Bulk Size
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