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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   SMURF1 Antibody (C-term)   

SMURF1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - SMURF1 Antibody (C-term) AP2104B
    The anti-SMURF1 Pab (Cat. #AP2104b) is used in Western blot to detect SMURF1 in HL-60 cell lysate.
  • IHC-P - SMURF1 Antibody (C-term) AP2104B
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9HCE7
Other Accession A2A5Z6, Q9HAU4, Q9PUN2, Q9CUN6
Reactivity Human
Predicted Mouse, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 86114 Da
Antigen Region 711-740 aa
Additional Information
Gene ID 57154
Other Names E3 ubiquitin-protein ligase SMURF1, hSMURF1, 632-, SMAD ubiquitination regulatory factor 1, SMAD-specific E3 ubiquitin-protein ligase 1, SMURF1, KIAA1625
Target/Specificity This SMURF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 711-740 amino acids from the C-terminal region of human SMURF1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSMURF1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms KIAA1625
Function E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA.
Cellular Location Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side
Research Areas
Citations ( 0 )


SMURF1 is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. This protein interacts with receptor-regulated SMADs specific for the BMP pathway, SMAD1 and SMAD5, in order to trigger their ubiquitination and degradation and thereby their inactivation.


Tajima, Y., et al., J. Biol. Chem. 278(12):10716-10721 (2003).
Suzuki, C., et al., J. Biol. Chem. 277(42):39919-39925 (2002).
Ebisawa, T., et al., J. Biol. Chem. 276(16):12477-12480 (2001).
Zhu, H., et al., Nature 400(6745):687-693 (1999).
Lambris, J., et al., J. Immunol. Methods 27(1):55-59 (1979).

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$ 295.00
$ 99.00
Cat# AP2104B
(40 western blots)
Availability: In Stock
Bulk Size
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