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UBCE7IP4 Antibody (N-term)Purified Rabbit Polyclonal Antibody (Pab)

Country
United States
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Ordering Information
Catalog # Size Availability Price  
AP2108a 0.1 mg 400 ul In Stock $ 255.00 Add to cart
  • Specification
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  • Backgrounds

UBCE7IP4 Antibody (N-term) - Product info

ApplicationWB, IHC
  • Applications Legend:
  • W=Western Blotting
  • IP=Immunoprecipitation
  • IHC-P=Immunohistochemistry (Paraffin)
  • IF-IC=Immunofluorescence (Immunocytochemistry)
  • F=Flow Cytometry
Primary AccessionP50876
Other AccessionQ925F3, Q5RFV4
ReactivityHuman, Hamster
PredictedMouse, Zebrafish
Concentration0.25 mg/ml
IsotypeRabbit Ig
Calculated MW32890 Da

UBCE7IP4 Antibody (N-term) - Additional info

Gene ID 9781
Other Names
RNF144A; KIAA0161; RNF144; UBCE7IP4; Probable E3 ubiquitin-protein ligase RNF144A; RING finger protein 144A; UbcM4-interacting protein 4; Ubiquitin-conjugating enzyme 7-interacting protein 4
Target/Specificity
This UBCE7IP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human UBCE7IP4.
Dilution
WB~~1:100~500
IHC~~1:50~100
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions
UBCE7IP4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

UBCE7IP4 Antibody (N-term) - Protein Information

Name RNF144A
Synonyms KIAA0161, RNF144, UBCE7IP4
Function
E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (By similarity)
Cellular Location
Membrane; Single-pass membrane protein (Potential)

UBCE7IP4 Antibody (N-term) - Related products

AP2108a: UBCE7IP4 Antibody (N-term)

AP2108b: RNF144 (UBCE7IP4) Antibody (C-term)

RI14573: RNF144A predesign siRNA

DC07785: Human RNF144A cDNA Clone

BP2108a: UBCE7IP4 Antibody (N-term) Blocking Peptide

BP2108b: UBCE7IP4 Antibody (C-term) Blocking Peptide

UBCE7IP4 Antibody (N-term) - Application data

  • The anti-UBCE7IP4 Pab (Cat. #AP2108a) is used in Western blot to detect UBCE7IP4 in HeLa cell lysate (Lane 1) and CHO cell lysate (Lane 2).

  • Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

UBCE7IP4 Antibody (N-term) - Research Areas

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BACKGROUND

The protein encoded by this protein contains a RING finger, a motif known to be involved in protein-DNA and protein-protein interactions. The mouse counterpart of this protein has been shown to interact with Ube2l3/UbcM4, which is an ubiquitin-conjugating enzyme involved in embryonic development.

REFERENCES

Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002). Nagase, T., et al., DNA Res. 3(1):17-24 (1996).