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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   ZNF281 Antibody (Center)   

ZNF281 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - ZNF281 Antibody (Center) AP21118a
    Western blot analysis of lysates from Hela, A549, MCF-7 cell line, mouse liver, mouse small intestine tissue lysate(from left to right), using ZNF281 Antibody (Center)(Cat. #AP21118a). AP21118a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9Y2X9
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 96915 Da
Additional Information
Gene ID 23528
Other Names Zinc finger protein 281, GC-box-binding zinc finger protein 1, Transcription factor ZBP-99, Zinc finger DNA-binding protein 99, ZNF281, GZP1, ZBP99
Target/Specificity This ZNF281 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 416-450 amino acids from the Central region of human ZNF281.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsZNF281 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ZNF281
Synonyms GZP1, ZBP99
Function Transcription repressor that plays a role in regulation of embryonic stem cells (ESCs) differentiation. Required for ESCs differentiation and acts by mediating autorepression of NANOG in ESCs: binds to the NANOG promoter and promotes association of NANOG protein to its own promoter and recruits the NuRD complex, which deacetylates histones. Not required for establishement and maintenance of ESCs (By similarity). Represses the transcription of a number of genes including GAST, ODC1 and VIM. Binds to the G- rich box in the enhancer region of these genes.
Cellular Location Nucleus.
Research Areas
Citations (0)

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Background

Transcription repressor that plays a role in regulation of embryonic stem cells (ESCs) differentiation. Required for ESCs differentiation and acts by mediating autorepression of NANOG in ESCs: binds to the NANOG promoter and promotes association of NANOG protein to its own promoter and recruits the NuRD complex, which deacetylates histones. Not required for establishement and maintenance of ESCs (By similarity). Represses the transcription of a number of genes including GAST, ODC1 and VIM. Binds to the G- rich box in the enhancer region of these genes.

References

Law D.J.,et al.Biochem. Biophys. Res. Commun. 262:113-120(1999).
Lisowsky T.,et al.FEBS Lett. 453:369-374(1999).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Gregory S.G.,et al.Nature 441:315-321(2006).
Zhang X.,et al.Nucleic Acids Res. 31:2900-2914(2003).

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$ 295.00
$ 99.00
Cat# AP21118a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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