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LIPI Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - LIPI Antibody (Center) AP21120a
    Western blot analysis of lysate from human testis tissue lysate, using LIPI Antibody (Center)(Cat. #AP21120a). AP21120a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q6XZB0
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 52922 Da
Antigen Region 224-258 aa
Additional Information
Gene ID 149998
Other Names Lipase member I, LIPI, 311-, Cancer/testis antigen 17, CT17, LPD lipase, Membrane-associated phosphatidic acid-selective phospholipase A1-beta, mPA-PLA1 beta, LIPI, LPDL
Target/Specificity This LIPI antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 224-258 amino acids from the Central region of human LIPI.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsLIPI Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LIPI
Synonyms LPDL
Function Hydrolyzes specifically phosphatidic acid (PA) to produce 2- acyl lysophosphatidic acid (LPA; a potent bioactive lipid mediator) and fatty acid. Does not hydrolyze other phospholipids, like phosphatidylserine (PS), phosphatidylcholine (PC) and phosphatidylethanolamine (PE) or triacylglycerol (TG).
Cellular Location [Isoform 1]: Cell membrane. Secreted Note=May associate with lipid draft.
Tissue Location Expressed in testis. Expressed exclusively at the connecting piece of the sperm.
Research Areas
Citations (0)
citation

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Background

Hydrolyzes specifically phosphatidic acid (PA) to produce 2-acyl lysophosphatidic acid (LPA; a potent bioactive lipid mediator) and fatty acid. Does not hydrolyze other phospholipids, like phosphatidylserine (PS), phosphatidylcholine (PC) and phosphatidylethanolamine (PE) or triacylglycerol (TG).

References

Hiramatsu T.,et al.J. Biol. Chem. 278:49438-49447(2003).
Wen X.-Y.,et al.Hum. Mol. Genet. 12:1131-1143(2003).
Hattori M.,et al.Nature 405:311-319(2000).

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$ 365.00
$ 140.00
Cat# AP21120a
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