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LHX5 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - LHX5 Antibody (Center) AP21158a
    Anti-LHX5 Antibody (Center) at 1:2000 dilution + rat brain lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9H2C1
Reactivity Rat
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Additional Information
Other Names LIM/homeobox protein Lhx5, LIM homeobox protein 5, LHX5
Target/Specificity This LHX5 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 255-289 amino acids from the Central region of human LHX5.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsLHX5 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LHX5
Function Plays an essential role in the regulation of neuronal differentiation and migration during development of the central nervous system.
Cellular Location Nucleus.
Tissue Location Expressed in fetal brain and in various regions of the adult central nervous system including the spinal cord, the thalamus, and the cerebellum EMBL; AF291181; AAG36963.1; -; mRNA EMBL; BC109230; AAI09231.1; -; mRNA CCDS; CCDS9171.1; - RefSeq; NP_071758.1; NM_022363.2 UniGene; Hs.302029; - ProteinModelPortal; Q9H2C1; - SMR; Q9H2C1; - STRING; 9606.ENSP00000261731; - iPTMnet; Q9H2C1; - PhosphoSitePlus; Q9H2C1; - BioMuta; LHX5; - DMDM; 18202938; - EPD; Q9H2C1; - PaxDb; Q9H2C1; - PeptideAtlas; Q9H2C1; - PRIDE; Q9H2C1; - ProteomicsDB; 80526; - Ensembl; ENST00000261731; ENSP00000261731; ENSG00000089116 GeneID; 64211; - KEGG; hsa:64211; - UCSC; uc001tvj.2; human CTD; 64211; - DisGeNET; 64211; - EuPathDB; HostDB:ENSG00000089116.3; - GeneCards; LHX5; - HGNC; HGNC:14216; LHX5 HPA; HPA053908; - MIM; 605992; gene neXtProt; NX_Q9H2C1; - OpenTargets; ENSG00000089116; - PharmGKB; PA30367; - eggNOG; KOG0490; Eukaryota eggNOG; ENOG410YIJ3; LUCA GeneTree; ENSGT00760000118921; - HOGENOM; HOG000231630; - HOVERGEN; HBG006263; - InParanoid; Q9H2C1; - KO; K18493; - OMA; NYDFFPH; - OrthoDB; EOG091G0BSD; - PhylomeDB; Q9H2C1; - TreeFam; TF315442; - GenomeRNAi; 64211; - PRO; PR:Q9H2C1; - Proteomes; UP000005640; Chromosome 12 Bgee; ENSG00000089116; - CleanEx; HS_LHX5; - Genevisible; Q9H2C1; HS GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW GO; GO:0000981; F:RNA polymerase II transcription factor activity, sequence-specific DNA binding; ISA:NTNU_SB GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro GO; GO:0021846; P:cell proliferation in forebrain; IEA:Ensembl GO; GO:0021702; P:cerebellar Purkinje cell differentiation; IEA:Ensembl GO; GO:0021937; P:cerebellar Purkinje cell-granule cell precursor cell signaling involved in regulation of granule cell precursor cell proliferation; IEA:Ensembl GO; GO:0021879; P:forebrain neuron differentiation; IEA:Ensembl GO; GO:0021766; P:hippocampus development; IEA:Ensembl GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:Ensembl GO; GO:0021527; P:spinal cord association neuron differentiation; IEA:Ensembl GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-KW CDD; cd00086; homeodomain; 1 InterPro; IPR009057; Homeobox-like_sf InterPro; IPR017970; Homeobox_CS InterPro; IPR001356; Homeobox_dom InterPro; IPR001781; Znf_LIM Pfam; PF00046; Homeobox; 1 Pfam; PF00412; LIM; 2 SMART; SM00389; HOX; 1 SMART; SM00132; LIM; 2 SUPFAM; SSF46689; SSF46689; 1 PROSITE; PS00027; HOMEOBOX_1; 1 PROSITE; PS50071; HOMEOBOX_2; 1 PROSITE; PS00478; LIM_DOMAIN_1; 2 PROSITE; PS50023; LIM_DOMAIN_2; 2 2: Evidence at transcript level; Complete proteome; DNA-binding; Homeobox; LIM domain; Metal-binding; Nucleus; Reference proteome; Repeat; Transcription; Transcription regulation; Zinc CHAIN 1 402 LIM/homeobox protein Lhx5 /FTId=PRO_0000075789 DOMAIN 3 61 LIM zinc-binding 1. {ECO:0000255|PROSITE- ProRule:PRU00125} DOMAIN 62 125 LIM zinc-binding 2. {ECO:0000255|PROSITE- ProRule:PRU00125} DNA_BIND 180 239 Homeobox. {ECO:0000255|PROSITE- ProRule:PRU00108} SEQUENCE 402 AA; 44406 MW; E880138A1A3FCA2A CRC64; MMVHCAGCER PILDRFLLNV LDRAWHIKCV QCCECKTNLS EKCFSREGKL YCKNDFFRRF GTKCAGCAQG ISPSDLVRKA RSKVFHLNCF TCMVCNKQLS TGEELYVIDE NKFVCKDDYL SSSSLKEGSL NSVSSCTDRS LSPDLQDALQ DDPKETDNST SSDKETANNE NEEQNSGTKR RGPRTTIKAK QLETLKAAFA ATPKPTRHIR EQLAQETGLN MRVIQVWFQN RRSKERRMKQ LSALGARRHA FFRSPRRMRP LGGRLDESEM LGSTPYTYYG DYQGDYYAPG SNYDFFAHGP PSQAQSPADS SFLAASGPGS TPLGALEPPL AGPHAADNPR FTDMISHPDT PSPEPGLPGT LHPMPGEVFS GGPSPPFPMS GTSGYSGPLS HPNPELNEAA VW
Citations (0)

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Background

Plays an essential role in the regulation of neuronal differentiation and migration during development of the central nervous system.

References

Zhao Y.,et al.Gene 260:95-101(2000).

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$ 295.00
$ 99.00
Cat# AP21158a
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Quantity:
(40 western blots)
Availability: In Stock
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