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AMPH Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - AMPH Antibody (Center) AP21189c
    Anti-AMPH Antibody (Center) at 1:1000 dilution + human brain lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 76 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - AMPH Antibody (Center) AP21189c
    AP21189c staining AMPH in Human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P49418
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 76257 Da
Additional Information
Gene ID 273
Other Names Amphiphysin, AMPH, AMPH1
Target/Specificity This AMPH antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 336-370 amino acids from the Central region of human AMPH.
Dilution WB~~1:1000
IHC-P~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAMPH Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AMPH
Synonyms AMPH1
Function May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton.
Cellular Location Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Peripheral membrane protein; Cytoplasmic side Cytoplasm, cytoskeleton
Tissue Location Neurons, certain endocrine cell types and spermatocytes
Research Areas
Citations (0)
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Background

May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton.

References

David C.,et al.FEBS Lett. 351:73-79(1994).
Yamamoto R.,et al.Hum. Mol. Genet. 4:265-268(1995).
Floyd S.R.,et al.Mol. Med. 4:29-39(1998).
Scherer S.W.,et al.Science 300:767-772(2003).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

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$ 365.00
$ 140.00
Cat# AP21189c
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