- CITATIONS: 3
|Application ||WB, IHC-P, E|
|Calculated MW||17769 Da|
|Antigen Region||1-30 aa|
|Other Names||Ubiquitin/ISG15-conjugating enzyme E2 L6, Retinoic acid-induced gene B protein, RIG-B, UbcH8, Ubiquitin carrier protein L6, Ubiquitin-protein ligase L6, UBE2L6, UBCH8|
|Target/Specificity||This UBE2L6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human UBE2L6.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||UBE2L6 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the covalent attachment of ubiquitin or ISG15 to other proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Promotes ubiquitination and subsequent proteasomal degradation of FLT3.|
|Tissue Location||Present in natural killer cells (at protein level).|
Provided below are standard protocols that you may find useful for product applications.
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). UBE2L6 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to function in the E6/E6-ap-induced ubiquitination of p53.
Kumar, S., et al., J. Biol. Chem. 272(21):13548-13554 (1997).
Ardley, H.C., et al., Cytogenet. Cell Genet. 89 (1-2), 137-140 (2000).
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