|Application ||WB, E|
|Other Accession||Q9D1C1, Q4R9D1, Q32PA5|
|Predicted||Mouse, Monkey, Bovine|
|Calculated MW||19652 Da|
|Antigen Region||142-172 aa|
|Other Names||Ubiquitin-conjugating enzyme E2 C, UbcH10, Ubiquitin carrier protein C, Ubiquitin-protein ligase C, UBE2C, UBCH10|
|Target/Specificity||This UBE2C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 142-172 amino acids from the C-terminal region of human UBE2C.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||UBE2C Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.|
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Provided below are standard protocols that you may find useful for product applications.
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2C is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression.
Okamoto, Y., et al., Cancer Res. 63(14):4167-4173 (2003).
Lin, Y., et al., J. Biol. Chem. 277(24):21913-21921 (2002).
Townsley, F.M., et al., Proc. Natl. Acad. Sci. U.S.A. 94(6):2362-2367 (1997).
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