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(Mouse) Smad1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IHC-P - (Mouse) Smad1 Antibody (Center) AP21203c
    AP21203c staining (Mouse) Smad1 in Mouse heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • WB - (Mouse) Smad1 Antibody (Center) AP21203c
    Anti-Smad1 Antibody (Center) at 1:1000 dilution + mouse skeletal muscle lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P70340
Reactivity Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 52157 Da
Additional Information
Gene ID 17125
Other Names Mothers against decapentaplegic homolog 1, MAD homolog 1, Mothers against DPP homolog 1, Dwarfin-A, Dwf-A, Mothers-against-DPP-related 1, Mad-related protein 1, mMad1, SMAD family member 1, SMAD 1, Smad1, Smad1, Madh1, Madr1
Target/Specificity This Mouse Smad1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 154-187 amino acids from the Central region of Mouse Smad1.
Dilution IHC-P~~1:25
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions(Mouse) Smad1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Smad1
Synonyms Madh1, Madr1
Function Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD) (By similarity). May play a role in the initiation and maintenance of spermatogenesis. SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1 (By similarity). May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression (PubMed:15329343).
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:Q15797}. Nucleus {ECO:0000250|UniProtKB:Q15797}. Note=In the cytoplasm in the absence of ligand. Migration to the nucleus when complexed with SMAD4. Colocalizes with LEMD3 at the nucleus inner membrane {ECO:0000250|UniProtKB:Q15797}
Tissue Location Ubiquitous.
Research Areas
Citations (0)

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Background

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD) (By similarity). May play a role in the initiation and maintenance of spermatogenesis. SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1 (By similarity). May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene [removed]PubMed:15329343).

References

Yingling J.M.,et al.Proc. Natl. Acad. Sci. U.S.A. 93:8940-8944(1996).
Zhao G.-Q.,et al.Mech. Dev. 61:63-73(1997).
Huang S.,et al.Gene 258:43-53(2000).
Carninci P.,et al.Science 309:1559-1563(2005).
Miura S.,et al.Mol. Cell. Biol. 20:9346-9355(2000).

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$ 295.00
$ 99.00
Cat# AP21203c
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(40 western blots)
Availability: In Stock
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