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(Mouse) Melk Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - (Mouse) Melk Antibody (C-term) AP21213b
    Anti-Melk Antibody (C-term)at 1:1000 dilution + mouse ovary lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 73 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - (Mouse) Melk Antibody (C-term) AP21213b
    Anti-Melk Antibody (C-term)at 1:2000 dilution + mouse spleen lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 73 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - (Mouse) Melk Antibody (C-term) AP21213b
    Anti-Melk Antibody (C-term) at 1:1000 dilution + mouse testis lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 73 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • FC - (Mouse) Melk Antibody (C-term) AP21213b
    Overlay histogram showing MCF-7 cells stained with AP21213b (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP21213b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession Q61846
Reactivity Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 72729 Da
Additional Information
Gene ID 17279
Other Names Maternal embryonic leucine zipper kinase, Protein kinase PK38, mPK38, Tyrosine-protein kinase MELK, Melk, Kiaa0175, Pk38
Target/Specificity This mouse Melk antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 434-468 amino acids from the C-terminal region of mouse Melk.
Dilution WB~~1:1000
FC~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions(Mouse) Melk Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Melk
Synonyms Kiaa0175, Pk38
Function Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis.
Cellular Location Cell membrane; Peripheral membrane protein
Tissue Location Expressed in testis, ovary, thymus, spleen and T- cell. Expressed by neural progenitors: highly enriched in cultures containing multipotent progenitors.
Research Areas
Citations (0)
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Background

Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis.

References

Gil M.,et al.Gene 195:295-301(1997).
Heyer B.S.,et al.Mol. Reprod. Dev. 47:148-156(1997).
Carninci P.,et al.Science 309:1559-1563(2005).
Okazaki N.,et al.DNA Res. 10:167-180(2003).
Church D.M.,et al.PLoS Biol. 7:E1000112-E1000112(2009).

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$ 365.00
$ 140.00
Cat# AP21213b
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