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UBE2G1 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - UBE2G1 Antibody (N-term) AP2121a
    Western blot analysis of anti-UBE2G1 Antibody (N-term) (Cat.#AP2121a) in mouse heart tissue lysates (35ug/lane). UBE2G1(arrow) was detected using the purified Pab.
    detail
  • WB - UBE2G1 Antibody (N-term) AP2121a
    Western blot analysis of UBE2G1 (arrow) using rabbit polyclonal UBE2G1 Antibody (R11) (Cat.#AP2121a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE2G1 gene.
    detail
  • IHC-P - UBE2G1 Antibody (N-term) AP2121a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P62253
Other Accession P62255, P62254, Q4R5Y8, NP_003333
Reactivity Human, Mouse
Predicted Monkey, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 19509 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 7326
Other Names Ubiquitin-conjugating enzyme E2 G1, E217K, UBC7, Ubiquitin carrier protein G1, Ubiquitin-protein ligase G1, Ubiquitin-conjugating enzyme E2 G1, N-terminally processed, UBE2G1, UBE2G
Target/Specificity This UBE2G1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human UBE2G1.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUBE2G1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name UBE2G1
Synonyms UBE2G
Function Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. May be involved in degradation of muscle-specific proteins. Mediates polyubiquitination of CYP3A4.
Tissue Location Widely expressed, mainly in skeletal muscle.
Research Areas
Citations (0)
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Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2G1 is a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 98-100% sequence identity with the zebrafish, frog, rat and mouse counterparts, which indicates that this enzyme is highly conserved in eukaryotes.

References

Watanabe, T.K., et al., Cytogenet. Cell Genet. 74 (1-2), 146-148 (1996).

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$ 182.50
$ 70.00
Cat# AP2121a
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