|Application ||WB, IF, IHC-P, FC, E|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||47773 Da|
|Target/Specificity||This mouse Shh antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 58-91 amino acids from the N-terminal region of mouse Shh.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||(Mouse) Shh Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior- posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Activates the transcription of target genes by interacting with its receptor PTCH1 to prevent normal inhibition by PTCH1 on the constitutive signaling activity of SMO.|
|Cellular Location||Sonic hedgehog protein C-product: Secreted, extracellular space. Note=The C- terminal peptide diffuses from the cell (PubMed:7891723)|
|Tissue Location||Expressed in a number of embryonic tissues including the notochord, ventral neural tube, floor plate, lung bud, zone of polarizing activity and posterior distal mesenchyme of limbs. In the adult, expressed in lung and neural retina|
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